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Merck
CN

51458

Urea

BioUltra, ≥99.5% (T)

Synonym(s):

Carbamide, Carbonyldiamide

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About This Item

Linear Formula:
NH2CONH2
CAS Number:
57-13-6
Molecular Weight:
60.06
EC Number:
200-315-5
UNSPSC Code:
12352200
MDL number:
MFCD00008022
Beilstein/REAXYS Number:
635724

Key Documents

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product line

BioUltra

assay

≥99.5% (T)

impurities

insoluble matter, passes filter test, ≤0.005% formaldehyde (HCHO), ≤0.05% biuret

ign. residue

≤0.01% (as SO4)

loss

≤0.05% loss on drying, 20 °C (HV)

pH

8.0-10.0 (25 °C, 8 M in H2O)

mp

132-135 °C (lit.)

solubility

H2O: 8 M at 20 °C, clear, colorless

density

1.335 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 mg/kg, sulfate (SO42-): ≤10 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

8 M in H2O

UV absorption

λ: 260 nm Amax: 0.03, λ: 280 nm Amax: 0.02

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

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Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Other Notes

Acetic acid -urea PAGE of proteins; In the extraction of mitochondrial complex V; Calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis; Tryptophan fluorescence in electron-transfer flavoprotein:ubiquinone oxidoreductase; Denaturation curve

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Resolution and reconstitution of complex V of the mitochondrial oxidative phosphorylation system: properties and composition of the membrane sector.
Y M Galante et al.
Archives of biochemistry and biophysics, 211(2), 643-651 (1981-10-15)
N J Watmough et al.
Biochemistry, 30(5), 1317-1323 (1991-02-05)
We have studied the intrinsic fluorescence of the 12 tryptophan residues of electron-transfer flavoprotein:ubiquinone oxidoreductase (ETF:QO). The fluorescence emission spectrum (lambda ex 295 nm) showed that the fluorescence is due to the tryptophan residues and that the contribution of the
B J Smith
Methods in molecular biology (Clifton, N.J.), 1, 63-73 (1984-01-01)
In SDS polyacrylamide gel electrophoresis, proteins are separated essentially on the basis of their sizes, by the sieving effect of the polyacrylamide gel matrix (see Chapter 6 ). In the absence of SDS, the proteins would still be subject to
Determination and analysis of urea and guanidine hydrochloride denaturation curves.
C N Pace
Methods in enzymology, 131, 266-280 (1986-01-01)
A Paulus et al.
Electrophoresis, 11(9), 702-708 (1990-09-01)
Polyacrylamide-filled gel columns are used to separate oligonucleotide samples. For homopolymeric standard samples, plots of migration time versus molecular size are presented over a range of 30-160 bases. With 2.5-4% T and 3.3% C gels, good resolution over the examined
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