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Merck
CN

51490

Hektoen Enteric Agar

NutriSelect® Plus, powder, selective and differential for Salmonella spp., for Shigella spp., pack of 500 g, suitable for microbiology

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171606
Manufacturer/tradename:
NutriSelect® Plus
Form:
powder
Suitability:
Shigella spp., selective and differential for Salmonella spp., selective and differential for bacteria (General Media)
Application(s):
clinical testing
environmental
food and beverages
water monitoring, microbiology

Key Documents

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sterility

non-sterile

form

powder

Quality Level

200

shelf life

limited shelf life, expiry date on the label

composition

acid fuchsin, 0.1 g/L , agar, 15.0 g/L , ammonium ferric citrate, 1.5 g/L , bile salts, 9 g/L , bromothymol blue, 0.065 g/L , lactose, 12 g/L , mixed peptone, 12 g/L , salicin, 2 g/L , sodium chloride, 5 g/L , sodium thiosulfate, 5 g/L , sucrose, 12 g/L , yeast extract, 3 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.5±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
water monitoring, microbiology

suitability

Shigella spp., selective and differential for Salmonella spp., selective and differential for bacteria (General Media)

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Application

Hektoen enteric agar is a type of primary standard media, which may be commonly used in the selective detection and isolation of Salmonella in food microbiology labs and in human stool specimens.

Preparation Note

Suspend 76.67 g in 1 litre distilled water and let it soak. Heat up by constant stirring until boiling. Cool to 55 - 60 °C and pour into sterile plates. Do not autoclave. The Medium is very thermolabile and thus overheating should be avoided.

Other Notes

Plating medium for the isolation of enteric pathogens; Isolation of Shigellae
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCN1581
BCCL8834
BCCG1674
BCCF4105
BCCD6192

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W I Taylor et al.
Applied microbiology, 21(1), 32-37 (1971-01-01)
Two enrichment broths and four plating media were compared for efficiency of detection of enteric pathogens from 1,597 stool specimens. Of 170 salmonellae isolated from the composite of all methods, direct streaking yielded but 54%, whereas enrichment in gram-negative broth
S. King et al.
Applied Microbiology, 16, 557-557 (1968)
Entis P
Food Microbiology: The Laboratory (2002)
Comparison of four chromogenic media and Hektoen agar for detection and presumptive identification of Salmonella strains in human stools
Perez.MJ, et al.
Journal of Clinical Microbiology, 41, 1130-1134 (2003)
S King et al.
Applied microbiology, 16(4), 579-581 (1968-04-01)
During this study, 2,855 stool specimens from patients at Cook County Hospital were cultured for enteric pathogens. Hektoen Enteric Agar (HE) was compared with E M B and S S Agars by replicate samplings with both direct and indirect methods.

Articles

Differentiation and ID Media for Salmonella

Salmonella, with 2,300+ serotypes, causes half of food-borne illnesses, often from dairy, poultry, and eggs.

沙门氏菌分离和鉴定培养基

沙门氏菌污染是全球食源性疾病的第二大成因。控制沙门氏菌的暴发是食品监管机构、餐馆和整个食品工业的一项重要任务。沙门氏菌家族包括2300多种血清型的细菌，但其中有两种类型，即肠炎沙门氏菌和鼠伤寒沙门氏杆菌，约占所有人类感染的一半。大多数沙门氏菌的暴发可以追溯到乳制品、家禽和肉制品，但沙门氏菌几乎可以在任何食物上生长。鸡、鸡蛋及其衍生产品的风险特别高。

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