57993
Neutral Red solution
suitable for microscopy
Synonym(s):
3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Toluylene red
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About This Item
Empirical Formula (Hill Notation):
C15H17ClN4
CAS Number:
Molecular Weight:
288.78
UNSPSC Code:
12171500
PubChem Substance ID:
MDL number:
shelf life
limited shelf life, expiry date on the label
suitability
suitable for microscopy
SMILES string
[Cl-].Cc1cc2N=C3C=C\C(C=C3Nc2cc1N)=[N+](/C)C
InChI
1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H
InChI key
PGSADBUBUOPOJS-UHFFFAOYSA-N
General description
Well developed granules of volutin (polyphosphate) may be seen in unstained wet preparations as round refractive bodies within the bacterial cytoplasm. With basic dye, they tend to stain more strongly than the rest of the bacterium, and with methylene blue solution (Cat. No. 01363) they stain metachromatically, a reddish purple color.
Application
Neutral Red, a histological vital stain, is used as a stain and counterstain. It is used to stain Golgi apparatus in cells and Nissi granules in neurons. As a pH sensitive dye, neutral red stains lysosomes red within cells.
Other Notes
Composition:
Neutral red 1.0 g/l, Acetic acid 2 ml/l in water
Neutral red 1.0 g/l, Acetic acid 2 ml/l in water
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Regulatory Information
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Juan C Stockert et al.
Acta histochemica, 114(8), 785-796 (2012-02-22)
Although MTT is widely used to assess cytotoxicity and cell viability, the precise localization of its reduced formazan product is still unclear. In the present study the localization of MTT formazan was studied by direct microscopic observation of living HeLa
L Ivanova et al.
Chemical research in toxicology, 25(8), 1662-1674 (2012-06-27)
Enniatins are cyclic hexadepsipeptidic mycotoxins with ionophoric, antibiotic, and insecticidal activity. Enniatin B (EnnB), the most important analogue, is produced by many Fusarium species and is a common contaminant in grain-based foods. The compound's cytotoxic potential has been shown in
Robim M Rodrigues et al.
Toxicology in vitro : an international journal published in association with BIBRA, 27(2), 760-767 (2012-12-25)
When in vitro test systems are evaluated for assessment of the toxicity of chemical compounds, particular efforts are made to mimic the in vivo reality as close as possible. Cellular models with appropriate metabolic competence, i.e. with the potency to
Cássio do Nascimento et al.
Microscopy research and technique, 76(2), 126-132 (2012-10-31)
The aim of this randomized crossover investigation was to assess the biofilm formation on two titanium and one zirconia substrates in relation to the topography and surface roughness of the materials. Twenty-four discs specimens for each evaluated material (Machined pure
Kun Guo et al.
Biosensors & bioelectronics, 47, 184-189 (2013-04-13)
This study introduces a novel and simple method to covalently graft neutral red (NR) onto carbon surfaces based on spontaneous reduction of in situ generated NR diazonium salts. Immobilization of neutral red on carbon surface was achieved by immersing carbon
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