61748
LB Broth
suitable for microbiology
shelf life
limited shelf life, expiry date on the label
composition
sodium chloride, 5 g/L , tryptone, 10 g/L , yeast extract, 5 g/L
final pH
7.2 (37 °C)
application(s)
microbiology
Quality Level
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Application
For the culture of E. coli K12 strains for the preparation of phage and plasmid DNA acc. to Miller (1987).
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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S Heber et al.
Biochimica et biophysica acta, 1129(1), 1-12 (1991-12-02)
Recombinant DNA and genetic techniques were used to construct Escherichia coli strains SOH92 [phi(cls-lacZ+)] and SOH93 [phi(cls-'lacZ)hyb]. beta-Galactosidase (116 kDa) synthesized by strain SOH92 was primarily present in the particulate fraction. Strain SOH92 produced about 20-fold more beta-galactosidase activity than
Daniel J Cohen et al.
PloS one, 4(9), e7086-e7086 (2009-09-19)
This paper presents a low-cost inkjet dosing system capable of continuous, two-dimensional spatiotemporal regulation of gene expression via delivery of diffusible regulators to a custom-mounted gel culture of E. coli. A consumer-grade, inkjet printer was adapted for chemical printing; E.
Avraham Be'er et al.
mBio, 2(3), e00069-e00011 (2011-06-02)
Natural habitats vary in available nutrients and room for bacteria to grow, but successful colonization can lead to overcrowding and stress. Here we show that competing sibling colonies of Paenibacillus dendritiformis bacteria survive overcrowding by switching between two distinct vegetative
Zachary L Fowler et al.
Applied and environmental microbiology, 75(18), 5831-5839 (2009-07-28)
Identification of genetic targets able to bring about changes to the metabolite profiles of microorganisms continues to be a challenging task. We have independently developed a cipher of evolutionary design (CiED) to identify genetic perturbations, such as gene deletions and
Practical aspects of preparing phage and plasmid DNA: growth, maintenance, and storage of bacteria and bacteriophage.
H Miller
Methods in enzymology, 152, 145-170 (1987-01-01)
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