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Merck
CN

61769

Lectin-Peroxidase Conjugate from Triticum vulgaris/horseradish

≥90% protein basis, peroxidase ~200 U/mg

Synonym(s):

Peroxidase-Lectin Conjugate from Horseradish/Triticum vulgaris, WGA-HRP, WGA-POD Conjugate, Wheat germ Agglutinin-Peroxidase Conjugate

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About This Item

UNSPSC Code:
12352204
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assay

≥90% protein basis

peroxidase activity

~200 U/mg

solubility

PBS: 1 mg/mL, clear to very faintly hazy, brownish-yellow

shipped in

wet ice

storage temp.

−20°C

General description

conjugate description: MBS-linkage (Cat. No. 63173)

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Analysis Note

further activities: agglutination activity: ≤40 μg protein/ml agglutinates a 2% suspension of human erythrocytes after 1 hour incubation at 25°C

Other Notes

1 U corresponds to the amount of enzyme which oxidizes 1 μmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C
In retrograde tracing procedures in neural tissues


Regulatory Information

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D Herve et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 9(11), 3699-3708 (1989-11-01)
A unilateral injection of 6-OHDA (6 microgram/1.5 microliter) was made into the fields of Forel in order to estimate the effects of the destruction of ascending dopaminergic (DA) pathways on the denervation supersensitivity of DA D1 receptors in the rat
J T Keller et al.
The Journal of comparative neurology, 290(2), 310-321 (1989-12-08)
The origin, density, and distribution of sympathetic nerve fibers in the supratentorial dura mater of the rat were examined in detail in the current study by using wheat germ agglutinin horseradish peroxidase (WGA-HRP) retrograde tracing procedures, glyoxylic acid-induced fluorescence, and
J G Rutherford et al.
Anatomy and embryology, 180(5), 485-496 (1989-01-01)
Bidirectional transport of lectin conjugated horseradish peroxidase was employed to investigate the relative distribution of the cerebellar and cortical connections of the nucleus of Darkschewitsch in the cat. Injection of horseradish peroxidase into the deep cerebellar nuclei produced terminal labeling