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Merck
CN

62642

Lucifer Yellow CH dilithium salt

≥85% (HPLC)

Synonym(s):

6-Amino-2,3-dihydro-1,3-dioxo-2-hydrazinocarbonylamino-1H-benz[d,e]isoquinoline-5,8-disulfonic acid dilithium salt

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About This Item

Empirical Formula (Hill Notation):
C13H9Li2N5O9S2
CAS Number:
67769-47-5
Molecular Weight:
457.25
EC Number:
267-047-9
UNSPSC Code:
12171500
MDL number:
MFCD00006922
Beilstein/REAXYS Number:
4644601

Key Documents

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assay

≥85% (HPLC)

fluorescence

λex 428 nm; λem 530 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

[Li+].[Li+].NNC(=O)NN1C(=O)c2cc(cc3c(N)c(cc(C1=O)c23)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C13H11N5O9S2.2Li/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2

InChI key

RPKCZJYDUKVMGF-UHFFFAOYSA-L

Application

A highly fluorescent dye, useful in marking nerve cells.

Other Notes

Highly fluorescent dye for staining neurons. Can be bound to tissue by conventional aldehyde-containing biological fixatives

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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H Riezman
Cell, 40(4), 1001-1009 (1985-04-01)
Yeast cells have been shown to internalize lucifer yellow CH by endocytosis. Internalization of the fluorescent dye is time-, temperature-, and energy-dependent, it is not saturable, and the dye is accumulated in the vacuole. Some of the yeast secretory mutants
W W Stewart
Nature, 292(5818), 17-21 (1981-07-02)
Lucifer dyes are intensity fluorescent 4-aminonaphthalimides which are readily visible in living cells at concentrations and levels of illumination at which they are nontoxic. Because of their low molecular weight they frequently pass from one cell to another; this widespread

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