63016
M-17 Agar
NutriSelect® Basic, powder, for Lactococcus spp., for Streptococcus spp., pack of 500 g, suitable for microbiology
Synonym(s):
M17 Lactococcal Agar
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About This Item
NACRES:
NA.74
UNSPSC Code:
41171605
Manufacturer/tradename:
NutriSelect® Basic
Form:
powder
Suitability:
Lactococcus spp., Streptococcus spp.
Application(s):
agriculture
environmental
food and beverages, microbiology
environmental
food and beverages, microbiology
sterility
non-sterile
Quality Segment
form
powder
shelf life
limited shelf life, expiry date on the label
composition
agar, 12.75 g/L , ascorbic acid, 0.5 g/L , casein peptone (tryptic), 2.5 g/L , disodium β-glycerophosphate pentahydrate, 19 g/L , magnesium sulfate hydrate, 0.25 g/L , meat extract, 5 g/L , meat peptone (peptic), 2.5 g/L , soyapeptone (papainic), 5 g/L , yeast extract, 2.5 g/L
packaging
pack of 500 g
manufacturer/tradename
NutriSelect® Basic
technique(s)
microbiological culture: suitable
final pH
7.1±0.2 (25 °C)
application(s)
agriculture
environmental
food and beverages, microbiology
suitability
Lactococcus spp., Streptococcus spp.
General description
M-17 Agar is a specialized culture medium designed to support the growth of lactic streptococci. It contains casein peptone, meat peptone, soya peptone, yeast extract, and meat extract, which provide carbonaceous and nitrogenous compounds, vitamin B complex, and other essential growth factors. Ascorbic acid stimulates the growth of lactic streptococci, while magnesium sulfate supplies essential ions. Disodium β-glycerophosphate pentahydrate enhances the buffering capacity of the medium, promoting the growth of lactic streptococci and selectively inhibiting lactobacilli. The medium can also be used to detect phage infection by the presence of large, distinct plaques in the host bacteria’s growth, but plaque assays require the absence of glycerophosphate, which prevents calcium precipitation necessary for bacteriophage activity.
Application
M-17 Agar media is proposed for the cultivation, detection and enumeration of Iactic streptococci in milk and dairy products. The M-17 media are superior to other comparable culture media for the cultivation of the fastidious species Streptococcus cremoris, Streptococcus diacetilactis and Streptococcus Iactis. It is also suitable for cultivation and maintenance of starter cultures for cheese and yogurt manufacturing. This medium helps in detecting streptococcal mutants which are lactose non-fermenters. M-17 Agar media may also be used for the differentiation of bacteriophages infecting lactic streptococci.
Features and Benefits
NutriSelect®Basic is a powdered culture media offering
- Qualitycontrol with growth promotion test according to industry standards
- Qualityassurance acc. to ISO 9001
- Cost-efficient powderedmedia
Preparation Note
Dissolve 50 g in 950 ml distilled water and sterilize by autoclaving at 121°C for 15 minutes. After cooling add 50 ml aseptic 10% lactose solution.
Other Notes
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
This product will be discontinued in the near future; however, stock is still available. The recommended replacement product is 1.15108 - M 17 agar acc. to TERZAGHI
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class
11 - Combustible Solids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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