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Merck
CN

63237

TB-Medium Base according to Loewenstein-Jensen

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Loewenstein-Jensen Medium (Base)

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171607

Key Documents

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Technical Service
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sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

L-asparagine, 2.25 g/L , magnesium citrate, 0.375 g/L , magnesium sulfate, 0.15 g/L , malachite green, 0.25 g/L , monopotassium phosphate, 1.5 g/L , potato flour, 18.75 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

application(s)

clinical testing
environmental
food and beverages
life science and biopharma
veterinary, microbiology

suitability

mycobacteria

Quality Level

200

Related Categories

Application

The selective Lowenstein Jensen Medium is used for isolation of Mycobacteria sp. from contaminated specimens. Egg supplement enhances Mycobacteria growth. Malachite green, penicillin* and nalidixic acid* inhibit Gram-negative and Gram-positive bacteria. (* included in the Gruft Supplement)

Preparation Note

Suspend 37,2 g in 600 ml of purified water containing 12 ml of glycerol (Cat. No. 49769). Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Cool to 45-50°C and, aseptically, add 1 L of homogenised sterile eggs and if required 4 vials of Gruft Mycobacterial Supplement (Cat. No. 51803). Dispense into sterile culture tubes and coagulate at 85°C for 45 minutes in slant position.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN4832
BCCL5425
BCCK3256
BCCJ6608
BCCH8316

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Articles

Detection & Molecular Identification of Mycobacteria

Identifying Mycobacterium species crucial due to varying clinical significance; some pathogenic, others not.

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