63237
TB-Medium Base according to Loewenstein-Jensen
suitable for microbiology, NutriSelect® Plus
Synonym(s):
Loewenstein-Jensen Medium (Base)
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About This Item
NACRES:
NA.85
UNSPSC Code:
41171607
sterility
non-sterile
Quality Segment
form
powder
shelf life
limited shelf life, expiry date on the label
composition
L-asparagine, 2.25 g/L , magnesium citrate, 0.375 g/L , magnesium sulfate, 0.15 g/L , malachite green, 0.25 g/L , monopotassium phosphate, 1.5 g/L , potato flour, 18.75 g/L
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
application(s)
clinical testing
environmental
food and beverages
life science and biopharma
veterinary, microbiology
suitability
mycobacteria
Application
The selective Lowenstein Jensen Medium is used for isolation of Mycobacteria sp. from contaminated specimens. Egg supplement enhances Mycobacteria growth. Malachite green, penicillin* and nalidixic acid* inhibit Gram-negative and Gram-positive bacteria. (* included in the Gruft Supplement)
Preparation Note
Suspend 37,2 g in 600 ml of purified water containing 12 ml of glycerol (Cat. No. 49769). Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Cool to 45-50°C and, aseptically, add 1 L of homogenised sterile eggs and if required 4 vials of Gruft Mycobacterial Supplement (Cat. No. 51803). Dispense into sterile culture tubes and coagulate at 85°C for 45 minutes in slant position.
Other Notes
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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