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Merck
CN

63690

2-Mercaptoethanol

≥99.0% (GC)

Synonym(s):

β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

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About This Item

Linear Formula:
HSCH2CH2OH
CAS Number:
60-24-2
Molecular Weight:
78.13
EC Number:
200-464-6
UNSPSC Code:
12352209
MDL number:
MFCD00004890
Beilstein/REAXYS Number:
773648

Key Documents

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vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

assay

≥99.0% (GC)

expl. lim.

18 %

concentration

14.3 M (pure liquid)

ign. residue

≤0.01%

refractive index

n20/D 1.500 (lit.), n20/D 1.501

bp

157 °C (lit.)

density

1.114 g/mL at 25 °C (lit.)

cation traces

Al: ≤0.5 mg/kg, Ba: ≤0.1 mg/kg, Bi: ≤0.1 mg/kg, Ca: ≤0.5 mg/kg, Cd: ≤0.05 mg/kg, Co: ≤0.02 mg/kg, Cr: ≤0.02 mg/kg, Cu: ≤0.02 mg/kg, Fe: ≤0.1 mg/kg, K: ≤0.5 mg/kg, Li: ≤0.1 mg/kg, Mg: ≤0.1 mg/kg, Mn: ≤0.02 mg/kg, Mo: ≤0.1 mg/kg, Na: ≤1 mg/kg, Ni: ≤0.05 mg/kg, Pb: ≤0.1 mg/kg, Sr: ≤0.1 mg/kg, Zn: ≤0.1 mg/kg

storage temp.

2-8°C

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

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Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

A reducing agent in fluorescent reaction of o-phthaldialdehyde and amino acids in alkaline medium; For the dissociation of proteins

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

target_organs

Liver,Heart

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

165.2 °F - closed cup

flash_point_c

74 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCCB1073

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I Barshad
Science (New York, N.Y.), 131(3405), 988-990 (1960-04-01)
Magnesium ions (Mg(++)) were shown to constitute a substantial percentage of the total exchangeable cations in acidified clay samples from various sources. This finding helps in solving several problems of long standing in the fields of soil chemistry, soil formation
H.T. Keutmann et al.
Analytical Biochemistry, 29, 175-175 (1966)
A fluorimetric assay for available lysine in proteins.
C C Goodno et al.
Analytical biochemistry, 115(1), 203-211 (1981-07-15)
R F Chen et al.
Biochimica et biophysica acta, 576(2), 440-455 (1979-02-26)
The fluorescence properties of the products formed by reaction of o-phthaldialdehyde with amino acids and their derivatives, in the presence of thiol compounds, have been studied. The emission spectra, quantum yields, and lifetimes depend on the primary amine and thiol
Fluorescence reaction for amino acids.
M Roth
Analytical chemistry, 43(7), 880-882 (1971-06-01)

Global Trade Item Number

SKUGTIN
B4527-100MG04061833429723
B4527-25MG04061833267806
B4527-5MG04061833429730

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