66759
Lactose sulfite medium
suitable for microbiology
Synonym(s):
LS
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About This Item
NACRES:
NA.21
UNSPSC Code:
41171607
shelf life
limited shelf life, expiry date on the label
composition
Cysteine hydrochloride, 0.3 g/L , Lactose monohydrate, 10 g/L , Pancreatic digest of casein, 5 g/L , Sodium chloride, 2.5 g/L , Yeast extract, 2.5 g/L
final pH
7.1±0.2 (25 °C)
application(s)
microbiology
Application
For the confirmation of Clostridium perfringens.
Preparation Note
Suspend 20,3 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense 8 ml portions into tubes with Durham gas collecting tubes for gas detection. Sterilize in autoclave at 121 ºC for 15 minutes. Before using add to each tube 0,5 ml of a 12 g/L solution of sodium metabisulfite and 0,5 ml of a solution of 10 g/L of ferric ammonium citrate. Both solutions have to be freshly prepared and sterilized.
Storage Class
11 - Combustible Solids
Regulatory Information
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