69813
Molecular Weight Marker for proteins
suitable for electrophoresis, prestained
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About This Item
UNSPSC Code:
12352200
MDL number:
quality
prestained
mol wt
Mr 27000-180000
technique(s)
electrophoresis: suitable
suitability
in accordance for electrophoresis test
storage temp.
2-8°C
General description
The electrophoretic mobilities of the maker proteins are altered by the attachment of dye. For precise molecular weight determinations on Western blots, biotinylated Molecular Weight Standard Mixture (Sigma No. KR225) is recommended. Dissolve content of one vial in 800 μl sample buffer (2x Laemmli buffer (Cat. No. 11337) and 8 M urea (Cat. No. 51458), 1:1)
Other Notes
Allows visual location of electrophoretically blotted lanes on nitrocellulose, nylon or PVDF
SDS-PAGE-Mr:
triosephosphate isomerase; prestained; from rabbit muscle: 26600; lactate dehydrogenase; prestained; from rabbit muscle: 36500; fumarase; prestained; from porcine heart: 48500; pyruvate kinase; prestained; from chicken muscle: 58000; fructose-6-phosphate kinase; prestained; from rabbit muscle: 84000; β-galactosidase; prestained; from E. coli: 116000; α2-macroglobulin; prestained; from human plasma: 180000
triosephosphate isomerase; prestained; from rabbit muscle: 26600; lactate dehydrogenase; prestained; from rabbit muscle: 36500; fumarase; prestained; from porcine heart: 48500; pyruvate kinase; prestained; from chicken muscle: 58000; fructose-6-phosphate kinase; prestained; from rabbit muscle: 84000; β-galactosidase; prestained; from E. coli: 116000; α2-macroglobulin; prestained; from human plasma: 180000
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V C Tsang et al.
Analytical biochemistry, 143(2), 304-307 (1984-12-01)
Prestained protein molecular weight standards allow easy, direct visual location of electrophoretically transblotted lanes on nitrocellulose. They also provide a simple and accurate means for calibrating the molecular weights of resolved bands. Commercial prestained protein molecular weight standards, however, appear
Eduardo L V Silveira et al.
Clinical and vaccine immunology : CVI, 15(8), 1292-1300 (2008-06-27)
Earlier studies have demonstrated in A/Sn mice highly susceptible to Chagas' disease protective immunity against lethal Trypanosoma cruzi infection elicited by vaccination with an open reading frame (ORF) expressed by amastigotes. In our experiments, we used this mouse model to
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
U K Laemmli
Nature, 227(5259), 680-685 (1970-08-15)
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