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Merck
CN

74030

Nitrotetrazolium Blue chloride

≥88% (T)

Synonym(s):

2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT

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About This Item

Linear Formula:
C40H30N10O6 · 2Cl
CAS Number:
298-83-9
Molecular Weight:
817.64
EC Number:
206-067-4
UNSPSC Code:
12171500
PubChem Substance ID:
329765398
Beilstein/REAXYS Number:
4115923
MDL number:
MFCD00012159

Key Documents

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InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

assay

≥88% (T)

form

solid

impurities

≤10% solvent (mainly ethanol or methanol)

solubility

methanol: 50 mg/mL, clear, very strongly yellow

storage temp.

2-8°C

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Other Notes

Enzyme substrate e.g. for the determination of glucose-6-phosphate dehydrogenase; Precipitating agent for fibrinogen

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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D S Negi et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 25(2), 149-154 (1977-02-01)
An improved procedure is reported for the histochemical localization of glucose-6-phosphate dehydrogenase in fresh-frozen sections of animal and plant tissues. Used in this procedure are an incubation medium and an inert high-molecular-weight polyvinyl alcohol as a stabilizer to check enzyme
Isolation of human fibrinogen using nitroblue tetrazolium (NBT).
V Vila et al.
Clinica chimica acta; international journal of clinical chemistry, 138(2), 215-219 (1984-04-13)
Hideyo Hirai et al.
Clinical & experimental metastasis, 31(8), 977-989 (2014-10-19)
To understand colon cancer metastasis, we earlier analyzed a mouse model that developed liver metastasis of cancer cells disseminated from the spleen. We suggested that CCR1(+) bone marrow (BM)-derived cells are recruited to the microenvironment of disseminated colon cancer cells
Pablo Gómez-Ochoa et al.
Veterinary immunology and immunopathology, 146(1), 97-99 (2012-03-01)
The nitroblue tetrazolium reduction test (NBT) is an assay based on the activation percentage of neutrophils in peripheral blood, that has been proposed for the follow up of canine leishmaniosis owing to the narrow relationship between the molecules involved in
Sheema Khan et al.
Oncotarget, 5(17), 7599-7609 (2014-10-04)
Pancreatic cancer has a poor prognosis due to late diagnosis and ineffective therapeutic multimodality. MUC13, a transmembrane mucin is highly involved in pancreatic cancer progression. Thus, understanding its regulatory molecular mechanisms may offer new avenue of therapy for prevention/treatment of
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