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Merck
CN

74030

Sigma-Aldrich

Nitrotetrazolium Blue chloride

≥88% (T)

Synonym(s):

2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT

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About This Item

Linear Formula:
C40H30N10O6 · 2Cl
CAS Number:
298-83-9
Molecular Weight:
817.64
Beilstein:
4115923
EC Number:
206-067-4
MDL number:
MFCD00012159
UNSPSC Code:
12171500
PubChem Substance ID:
329765398

Key Documents

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Assay

≥88% (T)

form

solid

impurities

≤10% solvent (mainly ethanol or methanol)

solubility

methanol: 50 mg/mL, clear, very strongly yellow

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

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Other Notes

Enzyme substrate e.g. for the determination of glucose-6-phosphate dehydrogenase; Precipitating agent for fibrinogen

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302,H315,H319,H335

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Isolation of human fibrinogen using nitroblue tetrazolium (NBT).
V Vila et al.
Clinica chimica acta; international journal of clinical chemistry, 138(2), 215-219 (1984-04-13)
D S Negi et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 25(2), 149-154 (1977-02-01)
An improved procedure is reported for the histochemical localization of glucose-6-phosphate dehydrogenase in fresh-frozen sections of animal and plant tissues. Used in this procedure are an incubation medium and an inert high-molecular-weight polyvinyl alcohol as a stabilizer to check enzyme
Pablo Gómez-Ochoa et al.
Veterinary immunology and immunopathology, 146(1), 97-99 (2012-03-01)
The nitroblue tetrazolium reduction test (NBT) is an assay based on the activation percentage of neutrophils in peripheral blood, that has been proposed for the follow up of canine leishmaniosis owing to the narrow relationship between the molecules involved in
Jaime M Ross
Journal of visualized experiments : JoVE, (57)(57), e3266-e3266 (2011-12-07)
Mitochondrial DNA (mtDNA) defects are an important cause of disease and may underlie aging and aging-related alterations (1,2). The mitochondrial theory of aging suggests a role for mtDNA mutations, which can alter bioenergetics homeostasis and cellular function, in the aging
N I Vera-Jimenez et al.
Fish & shellfish immunology, 34(5), 1216-1222 (2013-03-05)
The respiratory burst is an important feature of the immune system. The increase in cellular oxygen uptake that marks the initiation of the respiratory burst is followed by the production of reactive oxygen species (ROS) such as superoxide anion and
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