75349
Oligo dT-Cellulose
suitable for affinity chromatography
Synonym(s):
Oligothymidylic acid, immobilized on cellulose
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About This Item
UNSPSC Code:
12190000
MDL number:
technique(s)
affinity chromatography: suitable
capacity
~20 A260 units/g, material capacity (poly A)(pH 7.5 in 0.5 M NaCl, 0°C)
storage temp.
−20°C
General description
mixture of oligothymidylic acids (up to 18 nucleotides) covalently attached to cellulose via the terminal 5′-phosphoryl end by the method of P.T. Gilham, JACS 86, 4982 (1964)
Other Notes
Isolation of polyadenylated RNA; Purification of mRNA; Purifying RNA by column chromatography
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Purifying RNA by column chromatography.
N K Tanner
Methods in enzymology, 180, 25-41 (1989-01-01)
Effect of flow rate on the isolation of polyadenylated RNA on oligo(dT)-cellulose columns.
S Nadin-Davis et al.
Journal of biochemical and biophysical methods, 11(2-3), 185-189 (1985-08-01)
Nikolay E Shirokikh et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(31), 10738-10743 (2008-07-29)
Eukaryotic mRNAs in which a poly(A) sequence precedes the initiation codon are known to exhibit a significantly enhanced cap-independent translation, both in vivo and in cell-free translation systems. Consistent with high expression levels of poxviral mRNAs, they contain poly(A) sequences
High-efficiency cloning of full-length cDNA; construction and screening of cDNA expression libraries for mammalian cells.
H Okayama et al.
Methods in enzymology, 154, 3-28 (1987-01-01)
H Aviv et al.
Proceedings of the National Academy of Sciences of the United States of America, 69(6), 1408-1412 (1972-06-01)
A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution with buffers of low ionic strength. Biologically active
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