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Merck
CN

79889

Millipore

Brain Heart Infusion Broth

suitable for microbiology, ready to use media in the bottle

Synonym(s):

BHI Broth, Brain Heart Broth

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shelf life

limited shelf life, expiry date on the label

composition

beef heart (infusion from 250g), 5 g/L
calf brains (infusion from 200g), 12.5 g/L
disodium hydrogen phosphate, 2.5 g/L
D(+)-glucose, 2 g/L
peptone, 10 g/L
sodium chloride, 5 g/L

final pH

7.4±0.2 (25 °C)

application(s)

microbiology

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Application

Brain Heart Infusion Broth is used for the cultivation of fastidious bacteria (streptococci, pneumococci, meningococci, etc.) and also is recommended for the cultivation of pathogenic fungi. Growth of the accompanying bacterial flora can be almost completely suppressed by adding 20 I.U. penicillin and 40 μg streptomycin per mL culture medium.

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