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Merck
CN

84606

Sigma-Aldrich

SBFI-AM

≥98.0% (HPCE)

Synonym(s):

Sodium indicator SBFI-AM, Sodium-binding benzofuran isophthalate-AM

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About This Item

Empirical Formula (Hill Notation):
C56H58N2O23
CAS Number:
129423-53-6
Molecular Weight:
1127.06
MDL number:
MFCD00082576
UNSPSC Code:
12171500
PubChem Substance ID:
24888240

Key Documents

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Assay

≥98.0% (HPCE)

solubility

DMSO: soluble
methanol: soluble

fluorescence

λex 389 nm; λem 580 nm in 0.1 M phosphate pH 7.0 (esterase)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

COc1cc2cc(oc2cc1N3CCOCCOCCN(CCOCC3)c4cc5oc(cc5cc4OC)-c6ccc(cc6C(=O)OCOC(C)=O)C(=O)OCOC(C)=O)-c7ccc(cc7C(=O)OCOC(C)=O)C(=O)OCOC(C)=O

InChI

1S/C56H58N2O23/c1-33(59)72-29-76-53(63)37-7-9-41(43(21-37)55(65)78-31-74-35(3)61)49-23-39-25-51(67-5)45(27-47(39)80-49)57-11-15-69-16-12-58(14-18-71-20-19-70-17-13-57)46-28-48-40(26-52(46)68-6)24-50(81-48)42-10-8-38(54(64)77-30-73-34(2)60)22-44(42)56(66)79-32-75-36(4)62/h7-10,21-28H,11-20,29-32H2,1-6H3

InChI key

MWUYLQGOGOLRQS-UHFFFAOYSA-N

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Application

suitable for the detection of Na+

Other Notes

Cell permeant deriv. of fluorescent indicator for intracellular sodium

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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S Ito et al.
Journal of neuroscience methods, 75(1), 21-27 (1997-07-18)
Palytoxin was found to equilibrate sodium ions (Na+) across the cell membrane much faster than dose gramicidin, which has been frequently used to calibrate the intracellular Na+ concentration ([Na+]in), in cells treated with a Na+-sensitive fluorescent dye, sodium-binding benzofuran isophthalate
G P Amorino et al.
Cytometry, 21(3), 248-256 (1995-11-01)
Intracellular sodium concentration ([Na+]i) can be measured using SBFI with image analysis or spectrofluorometry. However, SBFI cannot be used with most flow cytometry systems, due to its requirement for dual excitation in the UV range. Recently a new sodium indicator
A Minta et al.
The Journal of biological chemistry, 264(32), 19449-19457 (1989-11-15)
Fluorescent indicators sensitive to cytosolic concentrations of free Na+ have been synthesized and characterized. They consist of a crown ether, 1,7-diaza-4,10,13-trioxacyclopentadecane, linked via its nitrogens to fluorophores bearing additional liganding centers. In the currently preferred dye, SBFI (short for sodium-binding

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