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85992

N-(N-Succinyl-L-phenylalanyl)-2-aminoacridone

≥98.0% (HPCE)

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About This Item

Empirical Formula (Hill Notation):
C26H23N3O5
CAS Number:
115930-64-8
Molecular Weight:
457.48
UNSPSC Code:
12352200
MDL number:
MFCD00674546
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assay

≥98.0% (HPCE)

solubility

DMF: soluble, DMSO: soluble, H2O: soluble

fluorescence

λex 398 nm; λem 440 nm in 0.1 M Tris pH 8.0 (fluoroescence decreases upon cleavage with α-chymotrypsin)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

OC(=O)CCC(=O)N[C@@H](Cc1ccccc1)C(=O)Nc2ccc3Nc4ccccc4C(=O)c3c2

Application

suitable as photometric substrate for α-chymotrypsin (linear increase in absorbance at 450 nm)

Analysis Note

In addition to the emission maximum at 440 nm, there is a lower maxima at 463 nm.

Other Notes

Substrate for the continuous assay of α-chymotrypsin at wavelengths 450/570nm

Regulatory Information

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J H Baustert et al.
Analytical biochemistry, 171(2), 393-397 (1988-06-01)
A direct and continuous kinetic method for the fluorometric determination of alpha-chymotrypsin and trypsin is described, and 2-aminoacridone (2-AA) is introduced as a promising new fluorophore in analytical biochemistry. N-Succinyl- and N-glutaryl-phenylalanine as well as N-benzoylarginine were coupled to 2-AA