88415
Thiazolyl Blue Tetrazolium Bromide
≥97.0% (enzymatic)
Synonym(s):
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide
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About This Item
Empirical Formula (Hill Notation):
C18H16BrN5S
CAS Number:
Molecular Weight:
414.32
EC Number:
206-069-5
UNSPSC Code:
12171500
PubChem Substance ID:
Beilstein/REAXYS Number:
4081397
MDL number:
InChI key
AZKSAVLVSZKNRD-UHFFFAOYSA-M
InChI
1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
SMILES string
[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4
assay
≥97.0% (enzymatic)
mp
195 °C (dec.) (lit.)
solubility
methanol: 5 mg/mL, clear
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Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm.
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
Other Notes
Review: Tetrazolium salts and formazans
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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