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Merck
CN

89640

Sigma-Aldrich

Toluidine Blue

suitable for microscopy, 8.74% (ZN (THEORY))

Synonym(s):

Tolonium chloride; Basic Blue 17

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About This Item

Empirical Formula (Hill Notation):
C15H16ClN3S · 0.5ZnCl2
CAS Number:
6586-04-5
Molecular Weight:
373.97
Colour Index Number:
52040
Beilstein:
4068052
MDL number:
MFCD03548293
UNSPSC Code:
12171500
PubChem Substance ID:
329769757
NACRES:
MA.02

Key Documents

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Quality Level

200

Assay

8.74% (ZN (THEORY))

form

powder or crystals

technique(s)

microbe id | staining: suitable

color

green to very dark green

solubility

methanol: 0.01 g/10 mL, blue to very deep blue

εmax

≥800 at 621-636 nm in water

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C.Cc4cc5N=C6C=CC(\C=C6Sc5cc4N)=[N+](\C)C

InChI

1S/2C15H15N3S.4ClH.Zn/c2*1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;;;;;/h2*4-8,16H,1-3H3;4*1H;/q;;;;;;+2/p-2

InChI key

DAQXTSXITPUGRG-UHFFFAOYSA-L

Related Categories

General description

Toluidine blue (C.l. 52040) is a diaminothiazine dye.It is also known as TBO, tolonium chloride, and toluidine blue O.

Application

Toluidine Blue is suitable for use in the following applications:
  1. to demonstrate amyloid; DNA in plant material, mast cells, Nissl substance, nuclei, and sulfatides
  2. in metachromatic staining of sulfated mucins and related materials
  3. as staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.
  4. staining of mucins in water-miscible resins and of nucleic acids in epoxy resins(1)

Biochem/physiol Actions

Toluidine Blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.It is found to have an affinity for nucleic acids and therefore binds to the nuclear material of tissues with a high DNA and RNA content.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK0876
BCCL0360
BCCH1582
BCCD9721
BCBZ9485

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A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone.
Zhu H, et al.
Nature Protocols, 5, 550-560 (2010)
Toluidine blue: A review of its chemistry and clinical utility.
Sridharan G and Shankar AA
International Journal of Oral and Maxillofacial Pathology, 16(2), 251-255 (2012)
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