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Merck
CN

910147

Fluorescein Azide

≥95%

Synonym(s):

4-((3-Azidopropyl)carbamoyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoesäure, 6-Carboxyfluorescein Azide, 6-FAM azide, N-(3-azidopropyl)-3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-6-carboxamide

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About This Item

Empirical Formula (Hill Notation):
C24H18N4O6
CAS Number:
1386385-76-7
Molecular Weight:
458.42
UNSPSC Code:
12352125
NACRES:
NA.22
MDL number:
MFCD18426596

Key Documents

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Product Name

Fluorescein Azide, ≥95%

SMILES string

[N+](=[N-])=NCCCNC(=O)c1cc(c(cc1)C(=O)O)C2=C3C(=CC(=O)C=C3)Oc4c2ccc(c4)O

InChI

1S/C24H18N4O6/c25-28-27-9-1-8-26-23(31)13-2-5-16(24(32)33)19(10-13)22-17-6-3-14(29)11-20(17)34-21-12-15(30)4-7-18(21)22/h2-7,10-12,29H,1,8-9H2,(H,26,31)(H,32,33)

InChI key

SJAGNCADRVABJA-UHFFFAOYSA-N

assay

≥95%

form

powder

reaction suitability

reaction type: click chemistry

storage temp.

−20°C

Related Categories

Application

This 488nm absorbing dye is probably the most popular dye in cell biology and represents an alternative to Alexa Fluor 488 and the DyLight 488. Fluorescent dye azides can be used for the fluorescent labeling of terminal alkyne-modified molecules by the Cu(I)-catalyzed azide-alkyne (CuAAC) reaction (click reaction).

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKCN9304
MKCM7954
MKCM3649

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Philipp M E Gramlich et al.
Angewandte Chemie (International ed. in English), 47(44), 8350-8358 (2008-09-25)
The attachment of labels onto DNA is of utmost importance in many areas of biomedical research and is valuable in the construction of DNA-based functional nanomaterials. The copper(I)-catalyzed Huisgen cycloaddition of azides and alkynes (CuAAC) has recently been added to
Click-click-click: single to triple modification of DNA.
Philipp M E Gramlich et al.
Angewandte Chemie (International ed. in English), 47(18), 3442-3444 (2008-04-03)

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