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Merck
CN

91365

Reinforced Clostridial Agar

NutriSelect® Plus, powder, nonselective for Clostridium spp., pkg of 500 g, suitable for microbiology

Synonym(s):

RCA, RCM Agar, RC Agar, RCM

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171607
Manufacturer/tradename:
NutriSelect® Plus
Form:
powder
Suitability:
nonselective for Bacteroides spp., nonselective for Clostridium spp.
Application(s):
agriculture
bioburden testing
cleaning products
clinical testing
cosmetics
environmental
food and beverages
personal care
veterinary, microbiology

Key Documents

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sterility

non-sterile

form

powder

Quality Level

200

shelf life

limited shelf life, expiry date on the label

composition

agar, 13.5 g/L , beef extract, 10 g/L , casein enzymatic hydrolysate, 10 g/L , L-cysteine hydrochloride, 0.5 g/L , dextrose, 5 g/L , sodium acetate, 3 g/L , sodium chloride, 5 g/L , starch soluble, 1 g/L , yeast extract, 3 g/L

packaging

pkg of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

6.8±0.2 (25 °C)

application(s)

agriculture
bioburden testing
cleaning products
clinical testing
cosmetics
environmental
food and beverages
personal care
veterinary, microbiology

suitability

nonselective for Bacteroides spp., nonselective for Clostridium spp.

Related Categories

General description

Reinforced Clostridial Agar (RCA) is a powdered microbiological culture medium designed for the cultivation and enumeration of Clostridia. It contains essential nutrients such as beef extract, casein enzymatic hydrolysate, yeast extract, and dextrose, which provide nitrogen, vitamins, amino acids, and carbon for microbial growth. Sodium acetate acts as a buffering agent, while L-cysteine hydrochloride serves as a reducing agent to support anaerobic conditions. The medium has a final pH of 6.8 ± 0.2 at 25°C and is non-selective for Clostridium spp. and Bacteroides spp.

Application

Reinforced Clostridial Agar is widely used in clinical testing, food and beverage microbiology for the cultivation and enumeration of Clostridia. It is suitable for anaerobic bacteria and is commonly employed in bioburden testing, agriculture, and personal care product microbiology.

Features and Benefits

NutriSelectn® Plus is a powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

Preparation Note

Suspend 51 g of Reinforced Clostridial Agar in 1000 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 10 lbs. Pressure (115°C) for 15 minutes.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCN2858
BCCL9934
BCCL1568
BCCK1991
BCCH0599

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Articles

Detection, Identification and Differentiation of Clostridium perfringens

Clostridium perfringens can cause contamination in undercooked or improperly sterilized canned foods and water. Learn to detect, identify, and differentiate this pathogen.

产气荚膜梭菌特性及检测

产气荚膜梭菌会导致未煮熟或未正确消毒的罐头食品和水受到污染。了解如何检测、鉴定和区分这种病原体。

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