919233
Sodium 2-(4-hexylphenyl)diazene-1-sulfonate
(0.5% solution in 25mM aqueous ammonium bicarbonate, pH 7.0), ≥95%
Synonym(s):
4-Hexylphenylazosulfonate, Azo
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About This Item
Empirical Formula (Hill Notation):
C12H17N2O3S · Na
CAS Number:
Molecular Weight:
292.33
MDL number:
UNSPSC Code:
12161503
NACRES:
NA.22
Quality Segment
assay
≥95%
form
liquid
refractive index
n
density
0.994 g/mL
storage temp.
2-8°C
SMILES string
[Na+].[S](=O)(=O)(\N=N\c1ccc(cc1)CCCCCC)[O-]
InChI
1S/C12H18N2O3S.Na/c1-2-3-4-5-6-11-7-9-12(10-8-11)13-14-18(15,16)17;/h7-10H,2-6H2,1H3,(H,15,16,17);/q;+1/p-1/b14-13+;
InChI key
GOFLQKOPOSFUCU-IERUDJENSA-M
Application
4-Hexylphenylazosulfonate (Azo) is a photocleavable anionic surfactant developed by the Ge lab for use in high-throughput top-down and bottom-up proteomics by mass spectrometry (MS). Initially, the reagent showed a breakthrough as an improved method for top-down analysis [Brown et al. 2019]. Top-down analysis looks at intact proteins, while bottom-up analysis uses digested proteins. Of the two, top-down analysis is preferable for detecting post-translational modifications and sequence variations. Bottom-up is more commonly used and is likely to detect more proteins. This reagent was also shown to work for sample extraction that can be used for both top-down and bottom-up analysis on the same sample to maximize data collection[Brown et al. 2020].
Surfactants are required for protein extraction especially for challenging classes of protein including membrane proteins[Brown et al. 2020] and extracellular matrix proteins[Knott et al. 2020], but surfactants suppress the signal in MS. While removing a surfactant will improve the signal, this can cause protein loss and degradation. Azo allows for easy removal since it can be rapidly degraded using ultraviolet irradiation (λmax = 305 nm). Using Azo in this way reduces protein loss and gives an improved signal. Please see the Ge lab publications for information on using this product [Brown et al. 2019, Brown et al. 2020, Knott et al. 2020, and Aballo et al. 2021].
Product can be used with our line of photoreactors: Including Penn PhD (Z744035) & SynLED 2.0 (Z744080)
Surfactants are required for protein extraction especially for challenging classes of protein including membrane proteins[Brown et al. 2020] and extracellular matrix proteins[Knott et al. 2020], but surfactants suppress the signal in MS. While removing a surfactant will improve the signal, this can cause protein loss and degradation. Azo allows for easy removal since it can be rapidly degraded using ultraviolet irradiation (λmax = 305 nm). Using Azo in this way reduces protein loss and gives an improved signal. Please see the Ge lab publications for information on using this product [Brown et al. 2019, Brown et al. 2020, Knott et al. 2020, and Aballo et al. 2021].
Product can be used with our line of photoreactors: Including Penn PhD (Z744035) & SynLED 2.0 (Z744080)
Legal Information
PCT/US2019/035447
Storage Class
10 - Combustible liquids
WGK
WGK 2
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