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D9805

Fast Blue B Salt

Name: Fast Blue B Salt
Brand: SIAL

Dye content ~95 %, Powder

Synonym(s):

o-Dianisidine bis(diazotized) zinc double salt, Azoic Diazo No. 48, DBB, Diazo Blue B, Naphthanil Diazo Blue B

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About This Item

Linear Formula:

C₁₄H₁₂N₄O₂Cl₂ · ZnCl₂

CAS Number:

14263-94-6

Molecular Weight:

475.47

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24894113

EC Number:

238-153-2

MDL number:

MFCD00075203

Beilstein/REAXYS Number:

1499067

Colour Index Number:

37235

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Product Name

Fast Blue B Salt, Dye content ~95 %

description

Technical grade

Quality Level

200

form

powder

composition

Dye content, ~95%

technique(s)

microbe id | staining: suitable

color

yellow to dark brown

mp

>300 °C (lit.)

solubility

H₂O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc(ccc1[N+]#N)-c2ccc([N+]#N)c(OC)c2

InChI

1S/C14H12N4O2.4ClH.Zn/c1-19-13-7-9(3-5-11(13)17-15)10-4-6-12(18-16)14(8-10)20-2;;;;;/h3-8H,1-2H3;4*1H;/q+2;;;;;+2/p-4

InChI key

GPPKNJIWDULNQH-UHFFFAOYSA-J

Related Categories

Clinical & Diagnostics

Hematology Stains

Histology Stains

Tissue Diagnostics

Application

Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens.
It has been used as a spraying agent in thin layer chromatography for the identification of separated components.
It has also been used for the determination of the lipase activity.

Biochem/physiol Actions

Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye. Similarly, fast blue B is also used for α- and β-glucosidase activity.

pictograms

GHS02,GHS08

signalword

Danger

hcodes

H242,H350

pcodes

P202 - P210 - P235 - P308 + P313 - P370 + P378 - P403

Hazard Classifications

Carc. 1B - Self-react. C

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

supp_hazards

EUH044

Storage Class

4.1A - Other explosive hazardous materials

Regulatory Information

监管及禁止进口产品

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Use of the rapid diazonium blue B test to differentiate clinically important species of Trichosporon and Geotrichum.

D L Oblack et al.

Sabouraudia, 21(3), 243-245 (1983-09-01)

Trichosporon isolates form pink to violet pigments following reaction with diazonium blue B which reliably differentiates them from Geotrichum species. The inoculum could be grown in static culture without diminishing the colour intensity. This modification facilitates use of the diazonium

Investigations into the cellular contribution to host tissue proteases and inhibitors in gingival crevicular fluid.

C N Kennett et al.

Journal of clinical periodontology, 24(6), 424-431 (1997-06-01)

Gingival crevicular fluid (GCF) was collected from chronic periodontitis patients using plastic micropipettes and coverslip smears stained with antibodies for leukocyte markers and Toluidine Blue for mast cells. The smears consisted of 70-80% granulocytes, 10-20% monocytes/macrophages, 5% mast cells and

Overcoming the bell-shaped dose-response of cannabidiol by using Cannabis extract enriched in cannabidiol.

Gallily R, et al.

Pharmacology & Pharmacy, 6, 75-75 (2015)

Evaluation of acid phosphatase as a confirmation test for Clostridium perfringens isolated from water.

Sartory DP, et al.

Letters in Applied Microbiology, 42, 418-418 (2006)

Partial characterization of pyloric-duodenal lipase of gilthead seabream (Sparus aurata).

Nolasco H, et al.

Fish Physiology and Biochemistry, 37, 43-43 (2011)

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Global Trade Item Number

SKU	GTIN
D9805-100G	04061838446015
D9805-10G	04061835347582

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