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L5382

Light Green SF Yellowish

crystalline

Synonym(s):

Acid Green 5

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About This Item

Linear Formula:
C37H34N2O9S3Na2
CAS Number:
5141-20-8
Molecular Weight:
792.85
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24896400
EC Number:
225-906-5
MDL number:
MFCD00012121
Beilstein/REAXYS Number:
5717828
Colour Index Number:
42095
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Product Name

Light Green SF Yellowish, powder or crystals

form

powder or crystals

Quality Level

100

technique(s)

titration: suitable

color

dark red to purple

mp

288 °C (dec.) (lit.)

solubility

water: 10 mg/mL, clear

εmax

>600 at 631-634 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)\C(=C3\C=CC(\C=C3)=[N+](/CC)Cc4cccc(c4)S([O-])(=O)=O)c5ccc(cc5)S([O-])(=O)=O

InChI

1S/C37H36N2O9S3.2Na/c1-3-38(25-27-7-5-9-35(23-27)50(43,44)45)32-17-11-29(12-18-32)37(31-15-21-34(22-16-31)49(40,41)42)30-13-19-33(20-14-30)39(4-2)26-28-8-6-10-36(24-28)51(46,47)48;;/h5-24H,3-4,25-26H2,1-2H3,(H2-,40,41,42,43,44,45,46,47,48);;/q;2*+1/p-2

InChI key

DGOBMKYRQHEFGQ-UHFFFAOYSA-L

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General description

Light green SF yellowish (LGSFY) is a disodium salt of an acid triphenylmethane dye. It is also known as Light Green, Lissamine Green SF, Food Green 2, Green No. 205, Acid Green, Acid Brilliant Green 5, and Acid Brilliant Green SF. LGSFY is an important component of Papanicolaou stains in addition to eosin Y and bismark brown Y. Light green SF yellowish is a histological stain for collagen and a permitted colorant for foodstuffs and beverages.

Application

Light green SF is generally used as a cytoplasmic counterstain with nuclear stains like safranine and Feulgen stain. In histopatology, it is used for collagen fiber staining in Masson′s trichrome, Papanicolaou cytological polychrome stains, and Twort stain for microorganisms in tissue sections. It is a constituent of polychrome staining method used for differentiating secretory cells of the pituitary gland. Light green SF also stains the cellulose cell walls of plant cells.

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS4921
SHBS3672
SHBR7429
SHBQ5068
SHBR2047

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Gurr E
Synthetic Dyes in Biology, Medicine And Chemistry (2012)
Osteogenic differentiation as a result of BMP-2 plasmid DNA based gene therapy in vitro and in vivo.
Wegman F
European Cells & Materials, 21, 230-230 (2011)
Modification of apolipoprotein(a) lysine binding site reduces atherosclerosis in transgenic mice.
Boonmark NW
The Journal of Clinical Investigation, 100, 558-558 (1997)
J S Drijver et al.
Acta cytologica, 27(6), 693-698 (1983-11-01)
The role of phosphotungstic acid as the main pH-controlling agent in Papanicolaou's EA stain was analyzed, including its effect on the competition between eosin Y and light green, the principal components of EA solutions, and this acid was compared with
S D McArthur et al.
The Journal of bone and joint surgery. British volume, 74(5), 668-672 (1992-09-01)
We describe a method which may be useful for the selection of samples for the study of early fibrillation in human articular cartilage. Blocks of cartilage and bone were cut post-mortem from the medial tibial condyles of 29 male and
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Global Trade Item Number

SKUGTIN
L5382-10G04061833958063
L5382-25G04061833958070
L5382-100G04061833958056

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