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Merck
CN

N7005

Neutral Red

Dye content ≥90 %, Powder

Synonym(s):

3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red

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About This Item

Empirical Formula (Hill Notation):
C15H17ClN4
CAS Number:
553-24-2
Molecular Weight:
288.78
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24897804
EC Number:
209-035-8
MDL number:
MFCD00012651
Beilstein/REAXYS Number:
3918740
Colour Index Number:
50040

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Product Name

Neutral Red, Dye content ≥90 %

form

powder

Quality Level

200

composition

Dye content, ≥90%

technique(s)

titration: suitable

color

brown to black

pH

6.8-8.0, red to yellow

mp

290 °C (dec.) (lit.)

solubility

water: 10 mg/mL, dark red

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1

InChI

1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H

InChI key

PGSADBUBUOPOJS-UHFFFAOYSA-N

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General description

Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.

Application

Neutral red has multiple staining applications that include its use as a nuclear stain, counterstain in the Gram procedure, either alone, or as a component of the Twort stain (a fast green-neutral red mixture). Neutral red staining is also a viability assessment method used for the detection of viable yeast cells in dandruff samples and of mussel larvae. Neutral red is also recommended for use in viability assessment as it is selectively absorbed by lysosomes and other acidic organelles in cells. It is also used for supravital staining of living blood cells.
Useful as an indicator for preparing neutral red paper, and as a biological stain.

Biochem/physiol Actions

Neutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes.

Analysis Note

Purified

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000538609
0000538609
0000431642
0000431642
MKCB1224V

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Global Trade Item Number

SKUGTIN
N7005-25G04061832343754
N7005-5G04061834117810
N7005-1G04061826116999

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