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Merck
CN

A3902

Bovine Serum Albumin

lyophilized powder, Vitamin B12 deficient

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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About This Item

CAS Number:
UNSPSC Code:
12352202
EC Number:
232-936-2
MDL number:
Biological source:
bovine
Assay:
≥98%
Form:
lyophilized powder
Technique(s):
ELISA: suitable
Impurities:
<1.0 μg/g Vitamin B12
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biological source

bovine

description

≤ 1.0ug/g vitamin B12 content

assay

≥98%

form

lyophilized powder

purified by

heat shock fractionation

origin

USA origin

technique(s)

ELISA: suitable

impurities

<1.0 μg/g Vitamin B12

loss

≤5%

pH

7

solubility

water: soluble (40 mg/ml)

suitability

suitable for (Suitable for use in vitamin B12 assays.)

UniProt accession no.

foreign activity

BT Virus, none detected, VSV Virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Application

For use in vitamin B12 assays.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.


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Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Peter Ellmark et al.
Methods in molecular biology (Clifton, N.J.), 439, 199-209 (2008-03-29)
Recent advances in antibody microarray technology have facilitated the development of multiplexed diagnostic platforms. Highly parallel antigen expression data obtained from these arrays allow disease states to be characterized using protein patterns rather than individual protein markers. The development of
Xun Wu et al.
Redox biology, 20, 46-59 (2018-10-08)
Endothelial dysfunction induced by hyperhomocysteinemia (HHcy) plays a critical role in vascular pathology. However, little is known about the role of endoplasmic reticulum (ER) redox homeostasis in HHcy-induced endothelial dysfunction. Here, we show that Hcy induces ER oxidoreductin-1α (Ero1α) expression
Marcos V A S Navarro et al.
PLoS biology, 9(2), e1000588-e1000588 (2011-02-10)
The bacterial second messenger bis-(3'-5') cyclic dimeric guanosine monophosphate (c-di-GMP) has emerged as a central regulator for biofilm formation. Increased cellular c-di-GMP levels lead to stable cell attachment, which in Pseudomonas fluorescens requires the transmembrane receptor LapD. LapD exhibits a



Global Trade Item Number

SKUGTIN
A3902-10G04061833693193