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Merck
CN

B4921

PhastGel® Blue R

Pre-measured tablets, readily soluble.

Synonym(s):

Brilliant Blue R, Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R

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About This Item

Empirical Formula (Hill Notation):
C45H44N3NaO7S2
CAS Number:
6104-59-2
Molecular Weight:
825.97
EC Number:
228-060-5
PubChem Substance ID:
24891803
UNSPSC Code:
12171500
Beilstein/REAXYS Number:
5718025
MDL number:
MFCD00041762

Key Documents

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InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

InChI key

NKLPQNGYXWVELD-UHFFFAOYSA-M

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

Quality Level

200

Application

Detects most proteins at 50-100 ng per band
PhastGel® Blue R has been used for the staining of protein bands after SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis). It has also been used for the staining of cell clones in the culture media plates.
For detection of protein bands following electrophoresis.

Preparation Note

One tablet makes 400 mL of a 0.1% staining solution.

Legal Information

PhastGel is a registered trademark of Cytiva

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
MKCF0442
MKCF0195
SLBQ5336V
SLBN0955V
SLBM7863V

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Pharmacokinetics and Biodistribution Study of 7A7 Anti-Mouse Epidermal Growth Factor Receptor Monoclonal Antibody and Its F(ab')(2) Fragment in an Immunocompetent Mouse Model.
Rabasa Capote A, et al.
ISRN Pharmacology, 2012, doi: 10-doi: 10 (2012)
Production of infectious HCV genotype 1b virus in cell culture using a novel Set of adaptive mutations.
Mori KI, et al.
BMC Microbiology, 16, 224-224 (2016)
Marina Piscopo et al.
Hearing research, 178(1-2), 89-94 (2003-04-10)
The factors controlling otoconia growth are not well known but it seems that the type of proteins contained in the otoconia regulates the initiation and/or the subsequent rates of crystal growth determining the morphology and the size of the final
Grit Nebrich et al.
Electrophoresis, 28(10), 1607-1614 (2007-04-21)
The availability of easy-to-handle, sensitive, and cost-effective protein staining protocols for 2-DE, in conjunction with a high compatibility for subsequent MS analysis, is still a prerequisite for successful proteome research. In this article we describe a quick and easy-to-use methodological
G Houen et al.
Electrophoresis, 18(5), 701-705 (1997-05-01)
A method for staining proteins on polyvinylidene difluoride membranes without using organic solvent is described. The method uses preblocking of the membrane with either Tween 20 or polyethylene glycol followed by staining with 0.01% Coomassie Brilliant Blue. No destaining of
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