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Merck
CN

CCW4109

MRS Agar

Solid culture medium for detection, isolation, and cultivation of lactobacilli and other lactic acid bacteria, tube capacity 20 mL, tube filling volume 17 mL, ready-to-use, box of 20 tubes, for Lactobacillus spp.

Synonym(s):

Lactobacillus Agar acc. to De Man, Rogosa and Sharpe, de MAN, ROGOSA and SHARPE Agar

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About This Item

NACRES:
NA.74
UNSPSC Code:
12352106

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form

solid

shelf life

12 mo. at 8‑25 °C

feature

ready-to-use

composition

agar, 14.0 g/L , dipotassium phosphate, 2.00 g/L , D-(+)-glucose, 20.0 g/L , magnesium acetate, 0.20 g/L , manganese sulfate, 0.05 g/L , meat extract, 8.00 g/L , peptone proteose, 10.0 g/L , polysorbate 80, 1.08 g/L , sodium acetate, 5.0 g/L , tri-ammonium citrate, 2.0 g/L , yeast extract, 4.0 g/L

packaging

box of 20 tubes

storage condition

(Tightly closed)

tube capacity

20 mL

tube filling volume

17 mL

color

yellowish

pH

7.0±0.2 (25 °C)

Quality Level

200

application(s)

food and beverages
microbiology

suitability

Lactobacillus spp.

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General description

MRS Agar is a medium used for the cultivation of lactobacilli. It is a modified medium based on the highly nutritious properties of tomato juice. The addition of magnesium, manganese, and acetate, together with polysorbate, provides an improved medium for the growth of lactobacilli, including very fastidious species such as Lactobacillus brevis and Lactobacillus fermentum. The quality of the peptones, in addition to the meat and yeast extracts, combine all the necessary growth factors that make MRS medium one of the best media for the cultivation of lactobacilli. As the selectivity of this medium is low and contaminants tend to grow, subculturing in a (double layer) solid medium and then in broth is recommended to increase selectivity. In many cases, growth is encouraged by incubation in a CO2-enriched atmosphere.

Application

MRS Agar is a medium is suitable for the detection, isolation, and cultivation of lactobacilli and other lactic acid bacteria from food and beverages.

Storage Class

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
117685

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