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Merck
CN

CCW4691

Millipore

MRS Agar

bottle capacity 250 mL, bottle filling volume 200 mL, closure type, Plastic screw cap, box of 10 bottles, for lactic acid bacteria

Synonym(s):

Lactobacillus Agar acc. to De Man, Rogosa and Sharpe, de MAN, ROGOSA and SHARPE Agar

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.74

Key Documents

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form

solid

Quality Level

200

shelf life

12 mo. at 8‑25 °C (limited shelf life, expiry date on the label)

feature

closure type Plastic screw cap
ready-to-use

composition

agar, 14.0 g/L
dipotassium phosphate, 2.00 g/L
D-(+)-glucose, 20.0 g/L
magnesium sulfate, 0.20 g/L
manganese sulfate, 0.05 g/L
meat extract, 8.00 g/L
peptone proteose, 10.0 g/L
polysorbate 80, 1.08 g/L
sodium acetate, 5.0 g/L
tri-ammonium citrate, 2.0 g/L
yeast extract, 4.0 g/L

packaging

box of 10 bottles

storage condition

(Tightly closed)

bottle capacity

250 mL

bottle filling volume

200 mL

color

yellow-brown

pH

6.2±0.2 (25 °C)

application(s)

food and beverages
microbiology

suitability

lactic acid bacteria

General description

MRS Agar is a medium used for the cultivation of lactobacilli. It is a modification of a medium based on the highly nutritious properties of tomato juice. The addition of magnesium, manganese, and acetate, together with polysorbate, provides an improved medium for the growth of lactobacilli, including very fastidious species such as Lactobacillus brevis and Lactobacillus fermentum. The quality of the peptones, in addition to the meat and yeast extracts, combine all the necessary growth factors that make MRS medium one of the best media for the cultivation of lactobacilli. As the selectivity of this medium is low and contaminants tend to grow, subculturing in a (double layer) solid medium and then in broth is recommended to increase selectivity. In many cases, growth is encouraged by incubation in a CO2-enriched atmosphere.

Application

MRS Agar is a medium is suitable for the detection, isolation, and cultivation of lactobacilli and other lactic acid bacteria from food and beverages.

Other Notes

  • 1 box with 10 bottles of 250 mL
  • Injectable cap: Plastic screw inner cap
Using syringe needles with a diameter greater than 0.8 mm is not recommended.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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