shipped in
dry ice
storage temp.
−20°C
Analysis Note
Quality
- Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing.
- Extensive PCR testing is performed to screen for a number of pathogens.
- Tested and found negative for bacteria, fungi, and mycoplasma.
- Protein concentrations determined by Lowry method.
- Endotoxin units measured by Limulus Amoebocyte Lysate assay.
- Matrigel Matrix gel stability is tested for a period of 14 days at 37°C.
- Biological activity is determined for each lot using a neurite outgrowth assay.
General description
Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Other Notes
Source:
Engelbreth-Holm-Swarm Mouse Tumor.
Formulation:
High concentration laminin/entactin complex frozen in Dulbecco′s PBS.
Engelbreth-Holm-Swarm Mouse Tumor.
Formulation:
High concentration laminin/entactin complex frozen in Dulbecco′s PBS.
Legal Information
Corning is a registered trademark of Corning, Inc.
Matrigel is a registered trademark of Corning, Inc.
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Muhammedin Deliorman et al.
Bio-protocol, 10(18), e3764-e3764 (2021-03-05)
This protocol describes a simple method to cryopreserve mammalian cells within filter papers as an alternative to conventional slow-freezing approach. The method involves treating paper fibers with fibronectin, using low concentrations of the cryoprotectant dimethyl sulfoxide (DMSO), and slow freezing
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