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Merck
CN

F0380000

Formylfolic acid

European Pharmacopoeia (EP) Reference Standard

Synonym(s):

N-[4-[[(2-Amino-1,4-dihydro-4-oxo-6-pteridinyl)methyl]formylamino]benzoyl]-L-glutamic acid

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About This Item

Empirical Formula (Hill Notation):
C20H19N7O7
CAS Number:
Molecular Weight:
469.41
UNSPSC Code:
41116107
NACRES:
NA.24
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InChI

1S/C20H19N7O7/c21-20-25-16-15(18(32)26-20)23-11(7-22-16)8-27(9-28)12-3-1-10(2-4-12)17(31)24-13(19(33)34)5-6-14(29)30/h1-4,7,9,13H,5-6,8H2,(H,24,31)(H,29,30)(H,33,34)(H3,21,22,25,26,32)/t13-/m0/s1

InChI key

UGWUWNVTCLDEOG-ZDUSSCGKSA-N

API family

folic acid

application(s)

pharmaceutical (small molecule)

format

neat

General description

This product is provided as delivered and specified by the issuing Pharmacopoeia. All information provided in support of this product, including SDS and any product information leaflets have been developed and issued under the Authority of the Issuing Pharmacopoeia. For further information and support please go to the website of the issuing Pharmacopoeia.

Application

Formylfolic acid EP Reference standard, intended for use in laboratory tests only as specifically prescribed in the European Pharmacopoeia.

Packaging

The product is delivered as supplied by the issuing Pharmacopoeia. For the current unit quantity, please visit the EDQM reference substance catalogue.

Other Notes

Sales restrictions may apply.

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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A convenient procedure for purification of thymidylate synthase from L1210 cells.
C K Banerjee et al.
Analytical biochemistry, 121(2), 275-280 (1982-04-01)
Johan D M Patring et al.
Journal of separation science, 29(6), 889-904 (2006-07-13)
Applicability of several alkyl-bonded silica stationary phases was tested for gradient RP-HPLC of folates under highly aqueous conditions. High retention of folates was achieved on alternative phases with enhanced polarity and classical phases with higher carbon content. Phases exhibiting polar
Achim Freisleben et al.
Journal of agricultural and food chemistry, 50(17), 4760-4768 (2002-08-09)
[2H4]Folic acid was synthesized by deuterating p-aminobenzoic acid, which was then coupled to glutamic acid and 6-formylpterin. Using [2H4]folic acid as starting component enabled the preparation of labeled vitamers tetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, and 10-formylfolate which were characterized by electrospray mass
D White et al.
Journal of pharmaceutical sciences, 81(12), 1204-1209 (1992-12-01)
A densitometric thin-layer chromatographic method for the analysis of process impurities in leucovorin calcium was developed and validated. Using this method, folic acid, N10-formyldihydrofolic acid, p-aminobenzoic acid, and N-(4-aminobenzoyl)-L-glutamic acid were monitored with a limit of detection of approximately 0.1%
A M Saleh et al.
British journal of cancer, 46(3), 346-353 (1982-09-01)
The metabolism of [2-14C]+[3', 5', 7, 9-3H] folic acid and [214C]+[3', 5', 7, 9-3H] 10-formylfolate was studied in hospital inpatients. Metabolites detected in the urine after folic acid feeding included the unchanged compound, other folates and a number of breakdown

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