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Merck
CN

F4897

Sigma-Aldrich

Fluo 3 pentaammonium salt

≥75% (HPLC)

Synonym(s):

Glycine, N-[2-[[[[2-[bis(carboxymethyl) amino]-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxyy]methyl]methyl]oxy]-4-methylphenyl]-N-(carboxymethyl)-pentaammonium salt

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About This Item

Empirical Formula (Hill Notation):
C36H30Cl2N2O13 · 5NH3
CAS Number:
339221-91-9
Molecular Weight:
854.69
MDL number:
MFCD00133325
UNSPSC Code:
12171500
PubChem Substance ID:
24894873

Key Documents

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Assay

≥75% (HPLC)

storage temp.

−20°C

SMILES string

N.OC(=O)CN(CC(O)=O)c1ccccc1OCCOc2cc(ccc2N(CC(O)=O)CC(O)=O)C3=C4C=C(Cl)C(=O)C=C4Oc5cc(O)c(Cl)cc35

InChI

1S/C35H28Cl2N2O13.H3N/c36-21-10-19-28(12-25(21)40)52-29-13-26(41)22(37)11-20(29)35(19)18-5-6-24(39(16-33(46)47)17-34(48)49)30(9-18)51-8-7-50-27-4-2-1-3-23(27)38(14-31(42)43)15-32(44)45;/h1-6,9-13,40H,7-8,14-17H2,(H,42,43)(H,44,45)(H,46,47)(H,48,49);1H3

InChI key

FNBIDSKDJJRWBQ-UHFFFAOYSA-N

Application

Cell impermeable fluorescent indicator of intracellular Ca2+; non-fluorescent in the absence of Ca2+, lower binding affinity allows measurement of higher peaks of Ca2+ transients than with FURA-2.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
046K1212
036K1696
036K1697
113K1349
107H1099

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J P Kao et al.
The Journal of biological chemistry, 264(14), 8179-8184 (1989-05-15)
Fluo-3, one member of a family of new fluorescent Ca2+ indicators excitable at wavelengths in the visible (Minta, A., Kao, J. P. Y., and Tsien, R. Y. (1989) J. Biol. Chem. 264, 8171-8178), has been tested in living cells. We

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