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Merck
CN

I3261

Indo 1-AM

93 - 100% purity

Synonym(s):

4-(6-Carboxy-2-indolyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester

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About This Item

Empirical Formula (Hill Notation):
C47H51N3O22
CAS Number:
112926-02-0
Molecular Weight:
1009.91
PubChem Substance ID:
24896026
UNSPSC Code:
12171500
MDL number:
MFCD00036955

Key Documents

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Product Name

Indo 1-AM, ~95% (HPLC)

InChI

1S/C47H51N3O22/c1-28-7-11-39(49(19-43(56)68-23-63-29(2)51)20-44(57)69-24-64-30(3)52)41(15-28)61-13-14-62-42-18-35(37-16-34-8-9-36(17-38(34)48-37)47(60)72-27-67-33(6)55)10-12-40(42)50(21-45(58)70-25-65-31(4)53)22-46(59)71-26-66-32(5)54/h7-12,15-18,48H,13-14,19-27H2,1-6H3

SMILES string

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc2cc(ccc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)-c3cc4ccc(cc4[nH]3)C(=O)OCOC(C)=O

InChI key

CAWBRCOBJNWRLK-UHFFFAOYSA-N

assay

~95% (HPLC)

fluorescence

λex 330 nm; λem 450 nm (with Calcium), λex 356 nm; λem 478 nm in methanol

storage temp.

−20°C

Quality Level

100

Application

Cell permeable fluorescent probe for Ca2+; lower affinity than FURA 2; gives a large shift in fluorescent emission from 482 nm to 398 nm upon Ca2+ binding.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
MKBW8742V
MKCF1669
MKCC4756
MKCC1633
MKBZ4230V

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Grundler W
Cytometry, 40, 219-219 (2000)
E Roux et al.
American journal of respiratory cell and molecular biology, 19(3), 437-444 (1998-09-09)
We have previously observed that acrolein administered ex vivo to isolated airways alters the subsequent airway responsiveness. To examine the cellular mechanisms involved in this alteration, we have studied the effect of acrolein exposure on calcium signaling in myocytes freshly
T Guenoun et al.
Anesthesiology, 92(2), 542-549 (2000-02-26)
Propofol is considered to be an anesthetic agent with few or no negative inotropic effects. This study evaluated a possible direct depressant effect of propofol on sarcoplasmic reticulum Ca2+ accumulation and cardiomyocytes. The effects of propofol on intracellular Ca2+ transients
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