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Merck
CN

I9274

Invertase from baker′s yeast (S. cerevisiae)

200-300 units/mg solid

Synonym(s):

β-D-Fructofuranosidase, β-D-Fructofuranoside fructohydrolase, Saccharase

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About This Item

CAS Number:
UNSPSC Code:
12164500
NACRES:
NA.21
EC Number:
232-615-7
MDL number:
EC Number:
Specific activity:
200-300 units/mg solid
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Quality Segment

specific activity

200-300 units/mg solid

technique(s)

photometry: suitable

application(s)

cannabis testing
food and beverages
general analytical

foreign activity

α-glucosidase (maltase), phosphoglucomutase, phosphoglucose isomerase ≤0.01%, 6-phosphogluconic dehydrogenase, α-galactosidase, β-galactosidase, and NADH oxidase ≤0.001%

storage temp.

2-8°C

General description

Invertase is encoded by the SUC2 (Saccharomyces cerevisiae S288C) gene. In yeast this enzyme exist in: glycosylated (secreted) and non-glycosylated (intracellular) form. The external invertase is composed of carbohydrate (50%) and 60,000 D subunits.

Application

Invertase from baker′s yeast (S. cerevisiae) has been used:
  • in liquid media (YES) and is used to determine the invertase activity
  • supplemented to the reaction mixture in the extraction and analysis of sugars and starch
  • for sucrose hydrolysis

Used in the production of confectionary foods and artificial honey.

Biochem/physiol Actions

Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.

Other Notes

One unit will hydrolyze 1.0 μmole sucrose to glucose and fructose per minute at pH 4.6 at 25 °C.


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Pictograms

Health hazard

Signal Word

Danger

Hazard Codes

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

WGK

WGK 3

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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