DOTAP chloride

DOTAP chloride in research is as a transfection reagent for the delivery of nucleic acids, such as DNA and RNA, into cells. DOTAP chloride, also known as 1,2-dioleoyl-3-trimethylammonium-propane chloride, is a cationic lipid that is often used to form liposomal complexes with nucleic acids, enabling their efficient delivery into cells. This makes it a valuable tool in molecular biology, gene expression studies, and the development of gene therapy techniques.

Advanced drug delivery

Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10 μg DOTAP per μg DNA and approximately 10-20 μg DOTAP per mL cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded.

Research grade products that can easily be scaled to meets GMP needs.