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Merck
CN

N15405

Nitrotetrazolium Blue chloride monohydrate

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About This Item

Empirical Formula (Hill Notation):
C40H30Cl2N10O6 · H2O · CH3OH
Molecular Weight:
867.69
UNSPSC Code:
12171500
PubChem Substance ID:
24897501
MDL number:
MFCD00150013

Key Documents

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InChI

1S/C40H30N10O6.CH4O.2ClH.H2O/c1-55-37-25-29(13-23-35(37)47-41-39(27-9-5-3-6-10-27)45(43-47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-42-40(28-11-7-4-8-12-28)46(44-48)32-17-21-34(22-18-32)50(53)54;1-2;;;/h3-26H,1-2H3;2H,1H3;2*1H;1H2/q+2;;;;/p-2

SMILES string

[Cl-].[Cl-].CO.[H]O[H].COc1cc(ccc1-[n+]2nc(-c3ccccc3)n(n2)-c4ccc(cc4)[N+]([O-])=O)-c5ccc(c(OC)c5)-[n+]6nc(-c7ccccc7)n(n6)-c8ccc(cc8)[N+]([O-])=O

InChI key

RAMYNPGXWWDCAB-UHFFFAOYSA-L

impurities

<8 wt. % methanol

mp

189 °C (dec.) (lit.)

λmax

259 nm

Application

Used in the detection of nucleic acid hybridization. Also used in the detection of ascorbate peroxidase activity in native gels by inhibition of reduction.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 2 - STOT SE 3

target_organs

Eyes,Central nervous system, Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCBN5850V
STBD2386V
STBD1571V
STBC9192V
STBC8759V

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E P Diamandis et al.
Journal of clinical laboratory analysis, 7(3), 174-179 (1993-01-01)
We have used a double-stranded DNA probe linked to the cystic fibrosis locus to detect a single-copy gene from varying amounts of genomic DNA, with Southern blot analysis. The DNA plasmid probe was labeled with either biotin or digoxigenin. Biotin
R Mittler et al.
Analytical biochemistry, 212(2), 540-546 (1993-08-01)
A method for the detection of ascorbate peroxidase activity in native electrophoretic gels is described. The assay is based on the ability of ascorbate peroxidase to prevent the ascorbate-dependent reduction of nitroblue tetrazolium in the presence of H2O2. The method

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