O1875
Orcinol monohydrate
≥99% purity (HPLC), powder
Synonym(s):
3,5-Dihydroxytoluene, 5-Methylresorcinol
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About This Item
Linear Formula:
CH3C6H3-1,3-(OH)2 · H2O
CAS Number:
Molecular Weight:
142.15
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
207-984-2
MDL number:
Beilstein/REAXYS Number:
1071903
Product Name
Orcinol monohydrate, colorimetric detection reagent
biological source
synthetic
Quality Level
assay
≥99% (HPLC)
form
powder
technique(s)
titration: suitable
color
white to light brown
bp
290 °C (lit.)
mp
56-58 °C (lit.)
solubility
H2O: 50 mg/mL
density
1.290 g/cm3
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
O.Cc1cc(O)cc(O)c1
InChI
1S/C7H8O2.H2O/c1-5-2-6(8)4-7(9)3-5;/h2-4,8-9H,1H3;1H2
InChI key
NBKPNAMTHBIMLA-UHFFFAOYSA-N
General description
Orcinol is a small-molecule natural phenol involved in the production of a dye called orcein used for the staining of cells and chromosomes. Orcinol monohydrate is usually used to prepare a colorimetric reagent known as Bial′s reagent for the determination of RNA, DNA, and hexoses.
Application
Orcinol monohydrate has been used to prepare Bial′s reagent for Bial′s orcinol assay for pentose sugars. It is also used in the preparation of orcinol solution suitable for staining TLC plates and, measuring rhamnolipids in orcinol dye assays. Orcinol monohydrate has been used for the RNA assay.
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Marjan De Mey et al.
Analytical biochemistry, 353(2), 198-203 (2006-03-21)
Recent developments in cellular and molecular biology require the accurate quantification of DNA and RNA in large numbers of samples at a sensitivity that enables determination on small quantities. In this study, five current methods for nucleic acid quantification were
A simultaneous estimation method of DNA and RNA by the orcinol reaction and a study on the reaction mechanism.
Y Endo
Journal of biochemistry, 67(5), 629-633 (1970-05-01)
A modified orcinol reaction for RNA determination.
M Kamali et al.
Clinical chemistry, 15(5), 390-392 (1969-05-01)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| O1875-5G | 04061834217770 |
| O1875-10G | 04061835347926 |
| O1875-100G | 04061834217763 |