S-Gal^®/LB Agar Blend, without IPTG

S-Gal^®/LB Agar Blend, without IPTG is designed for selection of recombinant bacterial clones with the lac⁺ phenotype.

S-Gal/LB Agar Blend, without IPTG is designed to be used in systems that do not require the inducer isopropyl β-D-thiogalactopyranoside (IPTG). It also gives the researcher the ability to control the level of inducer for systems that do require IPTG.

Suspend contents of one packet in 500 mL distilled or deionized water. Sterilize by autoclaving for 15 to 20 minutes at 121-124°C. For microwaving, heat suspended mix until initial boiling. Mix well. Heat for short intervals with mixing until agar component is in solution. Do not allow boiling for extended periods of time. Antibiotics should be added following autoclaving or microwaving, after cooling to 48-52°C.

Components (g/L)
Tryptone 10 g
Yeast extract 5 g
Sodium chloride 5 g
Agar 12 g
S-Gal 0.3 g
Ferric ammonium citrate 0.5 g

For researchers who don′t need (or want to control the amount of) inducer in their media.

Related Products: IPTG (Isopropyl β-D-thiogalactopyranoside, I6758), Ampicillin (A2804), Kanamycin (K0879), Chloramphenicol (C7795), Tetracycline (T8032)

S-GAL is a registered trademark of Merck KGaA, Darmstadt, Germany