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Merck
CN

T8533

o-Tolidine

≥95% purity, powder or chunks

Synonym(s):

3,3′-Dimethylbenzidine, 4,4′-Bianisidine

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About This Item

Linear Formula:
[-C6H3(CH3)-4-NH2]2
CAS Number:
119-93-7
Molecular Weight:
212.29
NACRES:
NA.47
PubChem Substance ID:
24900539
UNSPSC Code:
12171500
EC Number:
204-358-0
MDL number:
MFCD00014773
Beilstein/REAXYS Number:
2210640

Key Documents

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Product Name

o-Tolidine, ≥95%

SMILES string

Cc1cc(ccc1N)-c2ccc(N)c(C)c2

InChI

1S/C14H16N2/c1-9-7-11(3-5-13(9)15)12-4-6-14(16)10(2)8-12/h3-8H,15-16H2,1-2H3

InChI key

NUIURNJTPRWVAP-UHFFFAOYSA-N

assay

≥95%

form

powder or chunks

technique(s)

titration: suitable

color

white to light brown

mp

128-131 °C (lit.)

solubility

water: slightly soluble 1.3 g/L at 25 °C

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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General description

o-Tolidine is a diacidic dye which is sparingly soluble in water and easily soluble in ether and alcohol. On exposure to chlorine or bromine vapors, it forms an intense blue color. This forms the basis for detection of chlorine, nitrates and hydrogen peroxide in air or water. It turns yellow on reacting with free chlorine. o-Tolidine easily forms acid salts.

Application

o-Tolidine has also been used to determine the presence of laccase activity in T. versicolor fungal colonies.
Sensitive colorimetric reagent for gold and for free chlorine in water.

Disclaimer

May be carcinogenic.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

212.0 °F - closed cup

flash_point_c

100 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Regulatory Information

危险化学品
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Certificates of Analysis (COA)

Lot/Batch Number
MKDB2957
MKCZ0060
MKCX1219
MKCV7349
MKCS9398

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Leo M.L. Nollet
Handbook of Water Analysis (2013)
Purification and Characterization of Laccase from the
White Rot Fungus Trametes versicolor
Moon-Jeong
Journal of Microbiology (2005)
Wiley-VCH
Ullmann's Fine Chemicals null
Yunjung Kim et al.
FEMS microbiology letters, 284(2), 172-175 (2008-05-28)
A white-rot basidiomycete, Phlebia tremellosa, produced a laccase that showed increased activity during degradation of phthalates. A laccase was purified through the ion exchange chromatography and preparative gel electrophoresis, and the estimated molecular weight was 75 kDa. The optimum pH
K L Woods et al.
Alimentary pharmacology & therapeutics, 10(3), 333-337 (1996-06-01)
Mucosal bleeding is frequently used as a measure of gastric mucosal integrity. We compared the orthotolidine method for quantifying haemoglobin in gastric juice with a method based on measurement of fluorescent porphyrins extracted from haem. We also investigated whether acid
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