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W0625

Wright stain

Name: Wright stain
Brand: SIAL

≤4% purity (HPLC), powder

Synonym(s):

Eosin Methylene blue according to Wright

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About This Item

CAS Number:

68988-92-1

UNSPSC Code:

12171500

NACRES:

NA.47

MDL number:

MFCD00082143

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Properties

Product Name

Wright stain, suitable for blood stain

assay

≤4% (HPLC)

Quality Level

200

form

powder

concentration

≤4% (HPLC)

technique(s)

microbe id | staining: suitable

color

dark green

solubility

methanol: 10 mg/10 mL

suitability

suitable for blood stain

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[s]5c6c(nc7c5cc(cc7)N(C)C)cc[c](c6)=[N+](C)C.Brc1c2[o+]c3c(c(c2cc(c1O)Br)c4c(cccc4)C(=O)O)cc(c(c3Br)O)Br

InChI

1S/C20H8Br4O5.C16H18N3S/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13/h1-6H,(H2-,25,26,27,28);5-10H,1-4H3/q;+1/p+1

InChI key

AXIKDPDWFVPGOD-UHFFFAOYSA-O

Description

General description

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

Application

Wright′s Stain is mainly used for the staining of peripheral blood smears and bone marrow smears.

It is also used for G-banding and helps in the analysis of poorly spread metaphases.
It has been used to study the cytogenetics of human hematopoietic cells.
It has also been employed in the karyotyping of human embryonic stem cells.
It is also used in the staining of chromosomes.
It is used for the exudate analysis of white blood cell concentration.

Reported to be superior to May-Grunwald Stain in blood staining.

Features and Benefits

The purified dyes in Wright′s Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses.

Preparation Note

Prepared using polychromed (oxidized) methylene blue and eosin Y.

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pictograms

GHS07

signalword

Warning

hcodes

H302,H319

pcodes

P280 - P301 + P312 + P330 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia

Kim, et al.

Cell, 173 (2018)

Generation of FHL2 homozygous knockout lines from human embryonic stem cells by CRISPR/Cas9-mediated ablation

Chang, et al.

Stem Cell Research, 27 (2018)

Lillie, R.D.

Conn?s Biological Stains, 495-495 (1977)

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Global Trade Item Number

SKU	GTIN
W0625-100G	04061835349340
W0625-10G	04061832346663
W0625-25G	04061835349357