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C2432

Sigma-Aldrich

Chloroform

contains 100-200 ppm amylenes as stabilizer, ≥99.5%

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Synonym(s):
Methylidyne trichloride, Trichloromethane
Empirical Formula (Hill Notation):
CHCl3
CAS Number:
Molecular Weight:
119.38
Beilstein:
1731042
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.04

vapor density

4.1 (vs air)

Quality Level

vapor pressure

160 mmHg ( 20 °C)

Assay

≥99.5%

form

liquid

contains

100-200 ppm amylenes as stabilizer

technique(s)

RNA extraction: suitable

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.492 g/mL at 25 °C (lit.)

SMILES string

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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General description

Chloroform is a colorless, volatile chlorinated organic solvent whose vapors have a narcotic effect. The product contains amylene as a stabilizer, since chloroform is susceptible to degradation with time. Amylene acts as a scavenger of free radicals formed during chloroform decomposition.

Application

Chloroform has been used for the isolation of RNA from mammalian tissue.

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

易制毒化学品(2类)
危险化学品

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

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    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. What amount of amylenes are present in Product C2432, Chloroform, as a stabilizer?

    The concentration of amylenes is 100 - 300 ppm.

  7. Has Product C2432, Chloroform, been tested for DNase and RNase activity?

    This product has not been tested for DNase and RNase activity. However, it has been tested and found to be suitable for the extraction of nucleic acids based upon the evaluation of extracted DNA by agarose electrophoresis.

  8. How can Product C2432, Chloroform, be used to extract proteins from nucleic acids?

    A protocol for using phenol:chloroform or phenol:chloroform:isoamyl alcohol to extract proteins from nucleic acids is described in Molecular Cloning: A Laboratory Manual, vol. 3, pp E.3 - E.4 (1989).

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Ramos P et al.
Respiratory Research, 15, 54-54 (2014)
GC-MS-Based Analysis of Chloroform Extracted Suberin-Associated Root Waxes from Arabidopsis and Other Plant Species.
GC-MS-Based Analysis of Chloroform Extracted Suberin-Associated Root Waxes from Arabidopsis and Other Plant Species.
Kosma DK, et al.
Bio-protocol, 5(24) (2015)
Immune activation and target organ damage are consequences of hydrodynamic treatment but not delivery of naked siRNAs in mice.
Racz Z et al.
Nucleic Acid Therapeutics, 21, 215-215 (2011)
RNA Isolation from Synechocystis.
Mironov KS and. Los DA.
Bio-protocol, 5(6) (2015)
H Yamasaki et al.
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie, 322(2-3), 151-159 (1999-04-10)
Gap junctional intercellular communication (GJIC) is considered to play a key role in the maintenance of tissue independence and homeostasis in multicellular organisms by controlling the growth of GJIC-connected cells. Gap junction channels are composed of connexin molecules and, so

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