Nitrilase

Nitrilase belongs to the C-N hydrolase superfamily. It possesses the catalytic triad motifs: glutamate, lysine, and cysteine and are distributed in fungi, plants, and animals.The isoelectric point (pI) of this protein is 8.1. Native polyacrylamide gel electrophoresis (PAGE).

Nitrilase may be used for immobilization on acryloyl crosslinked cellulose dialdehyde (ACCD) for catalytic activity studies.

Nitrilases catalyze the liberation of ammonia and the formation of carboxylic acid from the nitriles. The oligomeric structural organization of nitrilase is crucial for its functionality. It displays broad substrate specificity.

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1 U corresponds to the amount of enzyme which liberates 1 μmol ammonia per minute at pH 7.2 and 25°C with the conversion of acrylonitrile to acrylic acid