Nitrilase

Name: Nitrilase
Brand: SIGMA

recombinant, expressed in E. coli, ≥2.0 U/mg

Synonym(s):

Nitrile aminohydrolase

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About This Item

CAS Number:

9024-90-2

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.5.5.1 (BRENDA, IUBMB)

MDL number:

MFCD04040969

Specific activity:

≥2.0 U/mg

Recombinant:

expressed in E. coli

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Properties

recombinant

expressed in E. coli

form

crystals, powder

specific activity

≥2.0 U/mg

mol wt

41 kDa

storage temp.

2-8°C

Description

General description

Nitrilase belongs to the C-N hydrolase superfamily. It possesses the catalytic triad motifs: glutamate, lysine, and cysteine and are distributed in fungi, plants, and animals.The isoelectric point (pI) of this protein is 8.1. Native polyacrylamide gel electrophoresis (PAGE).

Application

Nitrilase may be used for immobilization on acryloyl crosslinked cellulose dialdehyde (ACCD) for catalytic activity studies.

Biochem/physiol Actions

Nitrilases catalyze the liberation of ammonia and the formation of carboxylic acid from the nitriles. The oligomeric structural organization of nitrilase is crucial for its functionality. It displays broad substrate specificity.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

1 U corresponds to the amount of enzyme which liberates 1 μmol ammonia per minute at pH 7.2 and 25°C with the conversion of acrylonitrile to acrylic acid