08081401
MT-2
from human lymph
Synonym(s):
MT 2, MT2
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About This Item
NACRES:
NA.81
UNSPSC Code:
12352200
biological source
human lymph
form
liquid
growth mode
Non-adherent or adherent on poly-L-lysine treated plastic
karyotype
2n=46
morphology
Lymphoblast
products
Adult T-cell leukaemia virus (ALT; HTLV-1)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
-65 to -96°C
Biochem/physiol Actions
Derived from a 45 year old Japanese female with generalised lymphadenopathy and heptosplenomegaly (Adult T-cell Leukaemia) by co-culture of her peripheral leukocytes with male umbilical cord lymphocytes. The cells carry HTLV-1 and support the growth of HIV. The cells should be handled under laboratory containment level 3 conditions. MT-2 has a male karyotype and the presence of a Y chromosome has been confirmed by STR profiling at ECACC
Human T cell leukaemia
Not specified
Preparation Note
RPMI 1640 + 2 mM Glutamine + 10% Decomplemented Fetal Bovine Serum (FBS).
Seed cultures between 2-3x100,000 cells/ml. Saturation density 9 x 100,000 cells/ml; 5% CO2; 37 °C.
Other Notes
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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S Harada et al.
Science (New York, N.Y.), 229(4713), 563-566 (1985-08-09)
The human T-cell lines MT-2 and MT-4 carry the human T-cell leukemia virus type I (HTLV-I). When MT-2 and MT-4 were infected with HTLV-III, the probable etiologic agent of the acquired immune deficiency syndrome (AIDS), rapid cytopathogenic effects and cytotoxicity
S Harada et al.
Virology, 146(2), 272-281 (1985-10-30)
Early events in the infection of the human T-lymphotropic virus type-I (HTLV-I)-positive MT-4 cell line by the acquired immune deficiency syndrome (AIDS) retrovirus HTLV-III were investigated. The virus was adsorbed completely to the cells within 60 min incubation after inoculation
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