11610
Azo-Casein
protease substrate
Synonym(s):
Azocasein
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About This Item
biological source
bovine
technique(s)
electrophoresis: suitable
solubility
water: 5 mg/mL
ε (extinction coefficient)
32-38 at 440 nm in 0.1 M NaOH at 1% (1 cm)
storage temp.
2-8°C
General description
Chromogenic substrate for proteolytic activity; Digestion results in the formation of colored components, which are soluble in trichloroacetic acid and determined (after neutralization) in the range 366 to 450 nm.
Application
Suitable as substrate for proteolytic enzymes and useful for post-electrophoretic detection of proteases.
Biochem/physiol Actions
Can be used to induce secondary amyloidosis in mice, in studies of amyloid protein processing.
Other Notes
Sales restrictions may apply
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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D Vázquez Peyronel et al.
Electrophoresis, 16(10), 1894-1897 (1995-10-01)
Azocasein, diffused into gels after polyacrylamide gel electrophoresis, provides a useful substrate for simple and rapid protease detection. After electrophoresis, the gels are incubated in an azocasein solution, immersed in trichloroacetic acid and then treated with NaOH. Proteolytic zones appear
Pineapple cysteine endopeptidases.
A D Rowan et al.
Methods in enzymology, 244, 555-568 (1994-01-01)
Fanqi Wang et al.
Immunology, 159(1), 109-120 (2019-10-14)
Serpins are evolutionarily conserved serine protease inhibitors that are widely distributed in animals, plants and microbes. In this study, we reported the cloning and functional characterizations of two novel serpin genes, HlSerpin-a and HlSerpin-b, from the hard tick Haemaphysalis longicornis