12007C
Fetal Bovine Serum
Australia origin, γ-irradiated, sterile-filtered, suitable for cell culture
Synonym(s):
Fetal Calf Sera, Fetal Calf Serum, GI FBS, GI FCS, GI Fetal Bovine Sera, GI Fetal Bovine Serum, FBS, FCS, sera, serum
About This Item
sterile-filtered
biological source
bovine fetus
Quality Level
sterility
γ-irradiated
sterile-filtered
product line
FBS Premier
composition
Hemoglobin, ≤25 mg/dL
origin
Australia origin
technique(s)
cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
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Application
Preparation Note
Analysis Note
- Endotoxin and hemoglobin tested
- Tested for the presence of bacteria, virus, and mycoplasma
- Triple filtered with 0.1 micron membrane under aseptic conditions
Other Notes
Legal Information
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?
Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored refrigerated at 2-8°C. If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days. For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance. For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C. For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
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Articles
Find answers to questions (FAQs) about fetal bovine serum (FBS) that is gamma-irradiated by the SER-TAIN™ process. Request a sample of FBS at Sigma-Aldrich.com.
Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.
Protocols
Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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