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Merck
CN

12238C

Sigma-Aldrich

Calf Serum

Iron-Supplemented, from formula-fed bovine calves, sterile-filtered, USA origin, suitable for cell culture

Synonym(s):

bovine calf sera, bovine calf serum, calf sera, calf serum

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About This Item

MDL number:
MFCD00243108
UNSPSC Code:
12352207
NACRES:
NA.71
Biological source:
bovine (calf) (formula-fed)
Origin:
USA origin
Sterility:
γ-irradiated
sterile-filtered
Shipped in:
dry ice

Key Documents

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biological source

bovine (calf) (formula-fed)

Quality Level

500

sterility

γ-irradiated
sterile-filtered

composition

hemoglobin, ≤35 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

The animal serum is generally used as a supplement in basal media formulations for the optimal growth of various cell types in vitro. Iron-fortified bovine calf serum (IF-BCS) is obtained from 4 - 6 months old formula-fed calves. IF-BCS is preferred as a cost-effective substitute for fetal bovine serum (FBS) in several applications.

Application

Calf Serum has been used as a component in α-minimum essential medium (α-MEM) to grow MLO-Y4 (osteocyte cell line) cells. It has also been used to wash the blood cells of a male corn snake and a chicken to determine the genome size corn snake genome size.
Endotoxin tested

Preparation Note

Each lot is supplemented to a constant iron concentration.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Phyllis S Frisa et al.
PloS one, 4(9), e7064-e7064 (2009-09-19)
To obtain non-relative measures of cell proteins, purified preparations of the same proteins are used as standards in Western blots. We have previously quantified SV40 large T antigen expressed over a several fold range in different cell lines and correlated
Stephen Gadomski et al.
Cell stem cell, 29(4), 528-544 (2022-03-12)
The autonomic nervous system is a master regulator of homeostatic processes and stress responses. Sympathetic noradrenergic nerve fibers decrease bone mass, but the role of cholinergic signaling in bone has remained largely unknown. Here, we describe that early postnatally, a
Asier Ullate-Agote et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(42), 26307-26317 (2020-10-07)
Reptiles exhibit a spectacular diversity of skin colors and patterns brought about by the interactions among three chromatophore types: black melanophores with melanin-packed melanosomes, red and yellow xanthophores with pteridine- and/or carotenoid-containing vesicles, and iridophores filled with light-reflecting platelets generating
M Lichtenberg et al.
Cell death & disease, 2, e196-e196 (2011-08-26)
Leucine-rich repeat kinase 2 (LRRK2) mutations are the most common known cause of Parkinson's disease (PD). The clinical features of LRRK2 PD are indistinguishable from idiopathic PD, with accumulation of α-synuclein and/or tau and/or ubiquitin in intraneuronal aggregates. This suggests
Winco Wh Wu et al.
Virology journal, 6, 68-68 (2009-06-06)
Early in infection, the genome of the influenza A virus, consisting of eight complexes of RNA and proteins (termed viral ribonucleoproteins; vRNPs), enters the nucleus of infected cells for replication. Incoming vRNPs are imported into the nucleus of infected cells

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