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12238C

Sigma-Aldrich

Calf Serum

Iron-Supplemented, from formula-fed bovine calves, sterile-filtered, USA origin, suitable for cell culture

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Synonym(s):
bovine calf sera, bovine calf serum, calf sera, calf serum
MDL number:
MFCD00243108

biological source

bovine (calf) (formula-fed)

Quality Level

500

sterility

γ-irradiated
sterile-filtered

composition

hemoglobin, ≤35 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

The animal serum is generally used as a supplement in basal media formulations for the optimal growth of various cell types in vitro. Iron-fortified bovine calf serum (IF-BCS) is obtained from 4 - 6 months old formula-fed calves. IF-BCS is preferred as a cost-effective substitute for fetal bovine serum (FBS) in several applications.

Application

Calf Serum has been used as a component in α-minimum essential medium (α-MEM) to grow MLO-Y4 (osteocyte cell line) cells. It has also been used to wash the blood cells of a male corn snake and a chicken to determine the genome size corn snake genome size.

Testing Method

Endotoxin tested

Preparation Note

Each lot is supplemented to a constant iron concentration.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Phyllis S Frisa et al.
PloS one, 4(9), e7064-e7064 (2009-09-19)
To obtain non-relative measures of cell proteins, purified preparations of the same proteins are used as standards in Western blots. We have previously quantified SV40 large T antigen expressed over a several fold range in different cell lines and correlated
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The autonomic nervous system is a master regulator of homeostatic processes and stress responses. Sympathetic noradrenergic nerve fibers decrease bone mass, but the role of cholinergic signaling in bone has remained largely unknown. Here, we describe that early postnatally, a
M Lichtenberg et al.
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