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Merck
CN

14280

Sigma-Aldrich

BES buffered saline

BioUltra, Molecular Biology, 2x concentrate

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

Key Documents

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grade

Molecular Biology

product line

BioUltra

form

liquid

quality

2x concentrate

composition

BES, 10.7 g/L
NaCl, 16.0 g/L
Na2HPO4, 0.27 g/L

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

refractive index

n20/D 1.338

pH

7.1 (25 °C)

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.07
λ: 280 nm Amax: 0.02

application(s)

diagnostic assay manufacturing

Application

BES buffered saline can be used to study molecular biology, DNA and RNA purification, biological buffers and zwitterionic compounds. BES buffered saline has been used in a study that identified how to increase the transfection efficacy and subsequent long-term culture of resting human pancreatic duct epithelial cells.

Preparation Note

concentration (in H2O): 10.7 g/l BES, 16.0 g/l NaCl, 0.27 g/l Na2HPO4

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCJ0428
BCCF5414
BCCD4381
BCBZ7015
BCBW4573

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J. Sambrook et al.
Molecular Cloning: A Laboratory Manual, B10 (1989)
R Jesenofsky et al.
Pancreas, 17(3), 262-265 (1998-10-27)
Preparation of pancreatic duct epithelial cells from adult organs is possible by limited digestion and outgrowth of cells. These primary cells are mitotically active for only a short period. Therefore transfection with SV40 large-T antigen is one method to obtain
Karine Laulagnier et al.
Cellular and molecular life sciences : CMLS, 75(4), 757-773 (2017-09-29)
Amyloid beta peptide (Aβ), the main component of senile plaques of Alzheimer's disease brains, is produced by sequential cleavage of amyloid precursor protein (APP) and of its C-terminal fragments (CTFs). An unanswered question is how amyloidogenic peptides spread throughout the

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