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Merck
CN

22178

Cellulase from Aspergillus niger

powder, off-white, ~0.8 U/mg

Synonym(s):

1,4-(1,3:1,4)-β-D-Glucan 4-glucanohydrolase

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-734-4
MDL number:
EC Number:
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Product Name

Cellulase from Aspergillus niger, powder, off-white, ~0.8 U/mg

biological source

Aspergillus niger

form

powder

specific activity

~0.8 U/mg

color

off-white

storage temp.

2-8°C

Quality Level

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Application

Cellulase from Aspergillus niger has been used:
  • in the preparation of the standardized solution to determine absolute cellulase levels
  • in phosphate-citric acid buffer for activity assay
  • in non-starch polysaccharide degrading enzyme cocktail for in vitro hydrolysis of defatted rice bran and in the approach to improve the nutritive value of poultry feed

Biochem/physiol Actions

Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.

General description

Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes.

Other Notes

1 U corresponds to the amount of enzymes which liberates 1 μmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Information

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M.A. Sawsan et al.
Zentralblatt Fur Mikrobiologie, 143, 55-55 (1988)
Helena Fernandes et al.
Bioresource technology, 281, 277-285 (2019-03-03)
The macroalgae aquaculture industry has grown up in the last years, and new applications for macroalgae should be considered. In this work, sequential biological treatments as solid-state fermentation (SSF) by Aspergillus ibericus and enzymatic hydrolysis (EH) were applied to washed
The use of surrogate standards as a QA/QC tool for routine analysis of microplastics in sewage sludge.
Philipp, et al.
The Science of the Total Environment, 835, 155485-155485 (2022)
G. Okada
Methods in Enzymology, 160, 259-259 (1988)
María Dolores Ayala et al.
Animals : an open access journal from MDPI, 10(12) (2020-12-05)
A 90-d feeding trial was conducted in which five groups of gilthead seabream (11.96 g initial body weight) were fed with a microalgae-free diet (control group, C) or four diets containing the microalgae Nannochloropsis gaditana at two inclusion levels (2.5%

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