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24326C

SAFC

EX-CELL® 302 Serum-Free Medium for CHO Cells

without L-glutamine, without phenol red, without sodium bicarbonate, dry powder, suitable for cell culture

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MDL number:
MFCD01634638
NACRES:
NA.71

Quality Level

500

form

dry powder

technique(s)

cell culture | mammalian: suitable (suspension)

shipped in

ambient

storage temp.

2-8°C

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Application

EX-CELL® 302 is a serum-free medium, which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR- or Glutamine Synthetase, or GS System™ because it does not contain hypoxanthine, thymidine or L-glutamine. If EX-CELL 302 is to be used with CHO cells transformed using the GS selection system the media needs to be supplemented with GS Supplement 50X for the additional amino acids and nucelosides. For use with the DHFR- selection system we recommend the addition of 4 mM L-Glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kD. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Pluronic® F68 has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems.

Caution

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.

Quantity

Formulated to contain 21.2 grams of powder per liter of medium

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
GS System is a trademark of Lonza Group Ltd.
Pluronic is a registered trademark of BASF

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Regulatory Information

动植物来源培养基

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Sapan J Patel et al.
Electrophoresis, 38(3-4), 417-428 (2016-11-03)
The mechanisms underlying the aberrant growth and interactions between cells are not understood very well. The pre-B acute lymphoblastic leukemia cells directly obtained from an adult patient grow very poorly or do not grow at all at low density (LD)
Héla Kallel et al.
Journal of biotechnology, 95(3), 195-204 (2002-05-15)
We have carried out the adaptation of BHK-21 cells to two serum free (Ex Cell 520 and HyQ PF CHO) and three animal protein free media: Ex Cell 302, HyQ PF CHO MPS and Rencyte BHK. After a direct switch

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