32855
Eosin Methylene blue
according to May-Grünwald
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About This Item
UNSPSC Code:
12171500
NACRES:
MA.02
PubChem Substance ID:
MDL number:
Colour Index Number:
45380/52015
form
powder or crystals
Quality Level
quality
according to May-Grünwald
εmax
≥1000 at 635-655 nm in methanol, ≥650 at 512-532 nm in methanol
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
InChI
1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1
InChI key
NXBZXYJXNIFCRJ-UHFFFAOYSA-M
General description
Eosin has been used in combination with methylene blue, to detect carboxlic acid group in organic compounds. It gives a red or orange-coloured precipitate on treatment with carboxylic acid solution in water, acetone, alcohol, or dioxin. It is also used as an indicator for differentiation of the colon from the typhoid-dysentery group of bacteria.
Mixture of eosin and methylene blue stains for use in differential microbiological media to culture and identify pathogenic microorganisms, e.g., Neisseria sicca,, Acinetobacter calcoaceticus.
Application
Eosin methylene blue was used for staining cells, during optical microscopy studies, done for analysis of cell morphology.
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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The effect of concentration of dyes on differentiation of enteric bacteria on eosin-methylene-blue agar.
Journal of Bacteriology, 45 (5), 471-471 (1943)
Detection of carboxylic acid group in organic compounds with eosin, mercurochrome, and methylene blue.
Johar GS.
Fresenius' Zeitschrift fur Analytische Chemie, 260 (2), 122-123 (1972)
Oncosis is associated with exposure of phosphatidylserine residues on the outside layer of the plasma membrane: a reconsideration of the specificity of the annexin V/propidium iodide assay.
Lecoeur H
Cytometry, 44 (1), 65-72 (2001)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 32855-25G | 04061826717585 |