Key Documents

View All Documentation

35848

2′,7′-Dichlorofluorescein

Name: 2′,7′-Dichlorofluorescein
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90% (T)

Synonym(s):

DCF, Dichlorofluorescein

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):

C₂₀H₁₀Cl₂O₅

CAS Number:

76-54-0

Molecular Weight:

401.20

UNSPSC Code:

12171500

NACRES:

NA.32

PubChem Substance ID:

57649439

EC Number:

200-968-6

Beilstein/REAXYS Number:

58009

MDL number:

MFCD00005047

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

product line

BioReagent

Quality Level

100

assay

≥90% (T)

form

solid

mp

280 °C (dec.) (lit.)

fluorescence

λ_ex 504 nm; λ_em 529 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

SMILES string

Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

InChI key

VFNKZQNIXUFLBC-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Clinical & Diagnostics

Dry Dyes & Certified Stains

Tissue Diagnostics

General description

2′,7′-Dichlorofluorescein, also known as DCF is a fluorescent dye. The DCF fluorescence excitation is at 498nm with emission at 522nm. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.

Application

2′,7′-Dichlorofluorescein is used as fluorescent probe to measure ROS (Reactive Oxygen Species) for biological studies. It may also be used as a dip reagent for the fluorometric determination of vicinal diols, glycosides, and phenols.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Fluorescence densitometric method for the determination of gluconic and lactobionic acids ("sugar acids") in pharmaceutical preparations.

G Gübitz et al.

Journal of chromatography, 117(2), 337-343 (1976-02-18)

An in situ fluorimetric method has been developed for the quantitation of gluconic and lactobionic acids and their salts in tablet formulations. The method is based on glycol cleavage with lead tetraacetate followed by treatment with dichlorofluorescein. Calcium gluconate and

W.D. Pfeffer et al.

Journal of Chromatography A, 506, 401-401 (1990)

H. Watanabe et al.

Analytical Sciences, 2, 461-461 (1986)

Role of reactive oxygen species and Ca(2+) dissociation from the myofilaments in determination of Ca(2+) wave propagation in rat cardiac muscle.

Masahito Miura et al.

Journal of molecular and cellular cardiology, 56, 97-105 (2012-12-26)

Ca(2+) waves are initiated not only by Ca(2+) leak from the sarcoplasmic reticulum (SR), but also by Ca(2+) dissociation from the myofilaments in the myocardium with nonuniform contraction. We investigated whether contractile properties and the production of reactive oxygen species

2',7'-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy.

Xiuping Chen et al.

Free radical research, 44(6), 587-604 (2010-04-08)

Reactive oxygen species (ROS) are critically important chemical intermediates in biological studies, due to their multiple physiologically essential functions and their often pathologically deleterious effects. Consequently, it is vital that their presence in biological samples has to be quantifiable. However

View All Related Papers

Global Trade Item Number

SKU	GTIN
35848-5G	04061831812848
35848-25G	04061831812831

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service